NextSeq / NovaSeq uses two-colour chemistry, which means 4 bases (A/T/C/G) are represented by 2 colors of light. Green is T, red is C, yellow (green + red) is A, and None (no green, no red) is G. As the sequencing goes cycle by cycle, the green and red light signal strength become weaker. So more and more A/T/C will be mistakenly categorized as G in the tails of reads. And finally it results in polyG.
One open source tool can be used to trim polyG tails automatically for NextSeq / NovaSeq reads. This tool is fastp, which is available at: https://github.com/OpenGene/fastp
NextSeq / NovaSeq uses two-colour chemistry, which means 4 bases (A/T/C/G) are represented by 2 colors of light. Green is T, red is C, yellow (green + red) is A, and None (no green, no red) is G. As the sequencing goes cycle by cycle, the green and red light signal strength become weaker. So more and more A/T/C will be mistakenly categorized as G in the tails of reads. And finally it results in polyG.
One open source tool can be used to trim polyG tails automatically for NextSeq / NovaSeq reads. This tool is fastp, which is available at: https://github.com/OpenGene/fastp
take also a look here - https://sequencing.qcfail.com/articles/illumina-2-colour-chemistry-can-overcall-high-confidence-g-bases/
take also a look here - https://sequencing.qcfail.com/articles/illumina-2-colour-chemistry-can-overcall-high-confidence-g-bases/
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NextSeq / NovaSeq uses two-colour chemistry, which means 4 bases (A/T/C/G) are represented by 2 colors of light. Green is T, red is C, yellow (green + red) is A, and None (no green, no red) is G. As the sequencing goes cycle by cycle, the green and red light signal strength become weaker. So more and more A/T/C will be mistakenly categorized as G in the tails of reads. And finally it results in polyG.
One open source tool can be used to trim polyG tails automatically for NextSeq / NovaSeq reads. This tool is fastp, which is available at: https://github.com/OpenGene/fastp
NextSeq / NovaSeq uses two-colour chemistry, which means 4 bases (A/T/C/G) are represented by 2 colors of light. Green is T, red is C, yellow (green + red) is A, and None (no green, no red) is G. As the sequencing goes cycle by cycle, the green and red light signal strength become weaker. So more and more A/T/C will be mistakenly categorized as G in the tails of reads. And finally it results in polyG.
One open source tool can be used to trim polyG tails automatically for NextSeq / NovaSeq reads. This tool is fastp, which is available at: https://github.com/OpenGene/fastp
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