Home >
Community >
Why the molecular weight of my recombinant protein is lower than...
Upvote
24
Downvote
+ Sds-page
+ Protein purification techniques
+ Protein analysis
+ Protein expression
+ Recombinant protein expression and purification
Posted by
KENNETH ROTICH
Why the molecular weight of my recombinant protein is lower than...
Hi, i am trying to express my recombinant protein in BL21 E.coli. The vector is pCOLD I. i used 6-his-tag for purification. The predicted MW is 47.7kDa. However, the actual MW appears to be lower than the expected MW in SDS-PAGE.(The position of my recombinant protein is a little bit higher than 37kDa.)I wonder what might happen to it. Is it due to the premature termination in the translation process?Thanks in advance!
