Home >
Community >
10X low rate of correct barcodes was observed for the candidate chemistry choices for the input
Upvote
33
Downvote
+ Bioinformatics
Posted by
Martin J Pitt
10X low rate of correct barcodes was observed for the candidate chemistry choices for the input
Answer from @maximilian-press, converted from comments:
I can't comment on 10X data specifically, however, I suggest writing a
simple script or command that counts the frequency of each barcode,
and reports back the N (10? 20? 100?) most frequent ones, which will
allow you to investigate in more detail. This is what I have done in
the case of suspected bardode swaps or reverse complement issues.
Answer from @maximilian-press, converted from comments:
I can't comment on 10X data specifically, however, I suggest writing asimple script or command that counts the frequency of each barcode,and reports back the N (10? 20? 100?) most frequent ones, which willallow you to investigate in more detail. This is what I have done inthe case of suspected bardode swaps or reverse complement issues.
I have solve this problem, cause the combined barcode is differernt from the common 10x barcode. when mapping the genome, need to add-- chemistry ARC-v1 when cellranger count.
I have solve this problem, cause the combined barcode is differernt from the common 10x barcode. when mapping the genome, need to add-- chemistry ARC-v1 when cellranger count.
Answer from @maximilian-press, converted from comments:
Answer from @maximilian-press, converted from comments:
More
VOTE
I have solve this problem, cause the combined barcode is differernt from the common 10x barcode. when mapping the genome, need to add-- chemistry ARC-v1 when cellranger count.
I have solve this problem, cause the combined barcode is differernt from the common 10x barcode. when mapping the genome, need to add-- chemistry ARC-v1 when cellranger count.
More
VOTE
VOTE