if you now the stock concentration and the final concentration you can calculate dilution factor and therefore the volume to add yo the final solution.
if you now the stock concentration and the final concentration you can calculate dilution factor and therefore the volume to add yo the final solution.
Yes make the units the same. So convert 100 mg/mL into ug/mL = 100,000 ug/mL.
You will now see that your stock requires a 10000x dilution to reach 10 ug/mL and will require a 2000x dilution to reach 50 ug/mL.
You can use c1v1=c2v2 to determine the volumes required to perform the dilutions.
As an example for you 10 ug/mL stock C1 is 100,000 C2 is 10 V1 is volume of your stock, as an exmaple lets say you pipette 10 ul or 0.01 mL V2 is final volume Ex: (100,000)×(.01)/(10) = 100 mL Thus you would need to pipette 10 uL into 100 mL (technically 100 mL minus 10 uL).
Yes make the units the same. So convert 100 mg/mL into ug/mL = 100,000 ug/mL.
You will now see that your stock requires a 10000x dilution to reach 10 ug/mL and will require a 2000x dilution to reach 50 ug/mL.
You can use c1v1=c2v2 to determine the volumes required to perform the dilutions.
As an example for you 10 ug/mL stock C1 is 100,000 C2 is 10 V1 is volume of your stock, as an exmaple lets say you pipette 10 ul or 0.01 mL V2 is final volume Ex: (100,000)×(.01)/(10) = 100 mL Thus you would need to pipette 10 uL into 100 mL (technically 100 mL minus 10 uL).
Hi; Aaron Dhanda shows how to dilute your concentration. Also, you can use Endmemo (http://www.endmemo.com/sconvert/mg_mlug_ml.php) easily. Your stock solution 100.000 ug/ml; if you want 10 ug/ml take 1 ul from your stock solution and fulfil to 1ml. c1v1=c2v2 formula is the best dilution calculation.
Hi; Aaron Dhanda shows how to dilute your concentration. Also, you can use Endmemo (http://www.endmemo.com/sconvert/mg_mlug_ml.php) easily. Your stock solution 100.000 ug/ml; if you want 10 ug/ml take 1 ul from your stock solution and fulfil to 1ml. c1v1=c2v2 formula is the best dilution calculation.
Hi,
Check this online calculator.
https://www.physiologyweb.com/calculators/dilution_calculator_mass_per_volume.html
Hi,
Check this online calculator.
https://www.physiologyweb.com/calculators/dilution_calculator_mass_per_volume.html
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Dear Flavia
if you now the stock concentration and the final concentration you can calculate dilution factor and therefore the volume to add yo the final solution.
eg
stock solution 100mg/ml
1mg = 1000ug therefore 100mg/ml= 100.000ug/ml
if your would like a final 10ug/ul
dilution factor= [stock solution]/[final solution] = 100.000/10= 10.000
Now if you would like to prepare for example 200ml
final volume/dilution factor=volume stock solution
200ml/10.000= 0,02ml --> 20ul
in case you would like 200ml but at 50ug/ml than
dilution factor = 100.000/50= 2.000
then
200ml/200.000= 0,1ml --> 100ul
you need to acquire practicse with the convertion from the different units
eg 1ml= 1000ul
1g=1000mg=1.000-000ug and so on.
Because to compare the different number, all the numbers need to be in the same scale.
Ciao
Manuele
Dear Flavia
if you now the stock concentration and the final concentration you can calculate dilution factor and therefore the volume to add yo the final solution.
eg
stock solution 100mg/ml
1mg = 1000ug therefore 100mg/ml= 100.000ug/ml
if your would like a final 10ug/ul
dilution factor= [stock solution]/[final solution] = 100.000/10= 10.000
Now if you would like to prepare for example 200ml
final volume/dilution factor=volume stock solution
200ml/10.000= 0,02ml --> 20ul
in case you would like 200ml but at 50ug/ml than
dilution factor = 100.000/50= 2.000
then
200ml/200.000= 0,1ml --> 100ul
you need to acquire practicse with the convertion from the different units
eg 1ml= 1000ul
1g=1000mg=1.000-000ug and so on.
Because to compare the different number, all the numbers need to be in the same scale.
Ciao
Manuele
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Yes make the units the same. So convert 100 mg/mL into ug/mL = 100,000 ug/mL.
You will now see that your stock requires a 10000x dilution to reach 10 ug/mL and will require a 2000x dilution to reach 50 ug/mL.
You can use c1v1=c2v2 to determine the volumes required to perform the dilutions.
As an example for you 10 ug/mL stock
C1 is 100,000
C2 is 10
V1 is volume of your stock, as an exmaple lets say you pipette 10 ul or 0.01 mL
V2 is final volume
Ex:
(100,000)×(.01)/(10) = 100 mL
Thus you would need to pipette 10 uL into 100 mL (technically 100 mL minus 10 uL).
Someone correct me if im wrong pls..
Yes make the units the same. So convert 100 mg/mL into ug/mL = 100,000 ug/mL.
You will now see that your stock requires a 10000x dilution to reach 10 ug/mL and will require a 2000x dilution to reach 50 ug/mL.
You can use c1v1=c2v2 to determine the volumes required to perform the dilutions.
As an example for you 10 ug/mL stock
C1 is 100,000
C2 is 10
V1 is volume of your stock, as an exmaple lets say you pipette 10 ul or 0.01 mL
V2 is final volume
Ex:
(100,000)×(.01)/(10) = 100 mL
Thus you would need to pipette 10 uL into 100 mL (technically 100 mL minus 10 uL).
Someone correct me if im wrong pls..
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VOTE
Hi Flavia,
You may use the web tool to calculate
https://www.alfa-chemistry.com/solution-dilution-calculator.htm
Hi Flavia,
You may use the web tool to calculate
https://www.alfa-chemistry.com/solution-dilution-calculator.htm
More
VOTE
Hi;
Aaron Dhanda shows how to dilute your concentration. Also, you can use Endmemo (http://www.endmemo.com/sconvert/mg_mlug_ml.php) easily. Your stock solution 100.000 ug/ml; if you want 10 ug/ml take 1 ul from your stock solution and fulfil to 1ml. c1v1=c2v2 formula is the best dilution calculation.
Hi;
Aaron Dhanda shows how to dilute your concentration. Also, you can use Endmemo (http://www.endmemo.com/sconvert/mg_mlug_ml.php) easily. Your stock solution 100.000 ug/ml; if you want 10 ug/ml take 1 ul from your stock solution and fulfil to 1ml. c1v1=c2v2 formula is the best dilution calculation.
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VOTE
Generally 1 mg/ml = 1000 ug/ml
I would like to suggest you the following link for conversion:
https://www.convertunits.com/from/mg/ml/to/ug/ml
Generally 1 mg/ml = 1000 ug/ml
I would like to suggest you the following link for conversion:
https://www.convertunits.com/from/mg/ml/to/ug/ml
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VOTE