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Hello, could somebody explain the difference between relative...
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Kimo Khaled
Hello, could somebody explain the difference between relative...
Fluorescence intensity measurements are usually relative, unlike absorbance measurements, because they depend on many factors, such as the instrument settings, the geometry of the sample, the intensity of the exciting light, the sensitivity of the detector, and so forth. So fluorescence intensity measurements are reported as relative fluorescence units (RFU). The size of the numbers has no real meaning. They are arbitrary.
If you wish, you can normalize the fluorescence intensity to some comparator, such as the fluorescence of a reference dye, dividing the first by the second. This could be useful in some circumstances to make it easier to compare measurements made at different times or under different circumstances.
Fluorescence intensity measurements are usually relative, unlike absorbance measurements, because they depend on many factors, such as the instrument settings, the geometry of the sample, the intensity of the exciting light, the sensitivity of the detector, and so forth. So fluorescence intensity measurements are reported as relative fluorescence units (RFU). The size of the numbers has no real meaning. They are arbitrary.
If you wish, you can normalize the fluorescence intensity to some comparator, such as the fluorescence of a reference dye, dividing the first by the second. This could be useful in some circumstances to make it easier to compare measurements made at different times or under different circumstances.
Fluorescence intensity measurements are usually relative, unlike absorbance measurements, because they depend on many factors, such as the instrument settings, the geometry of the sample, the intensity of the exciting light, the sensitivity of the detector, and so forth. So fluorescence intensity measurements are reported as relative fluorescence units (RFU). The size of the numbers has no real meaning. They are arbitrary.
If you wish, you can normalize the fluorescence intensity to some comparator, such as the fluorescence of a reference dye, dividing the first by the second. This could be useful in some circumstances to make it easier to compare measurements made at different times or under different circumstances.
Fluorescence intensity measurements are usually relative, unlike absorbance measurements, because they depend on many factors, such as the instrument settings, the geometry of the sample, the intensity of the exciting light, the sensitivity of the detector, and so forth. So fluorescence intensity measurements are reported as relative fluorescence units (RFU). The size of the numbers has no real meaning. They are arbitrary.
If you wish, you can normalize the fluorescence intensity to some comparator, such as the fluorescence of a reference dye, dividing the first by the second. This could be useful in some circumstances to make it easier to compare measurements made at different times or under different circumstances.
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