DCF is diffusible from the cell, so if the preparation and assay take variable time, it will create inconsistent results among H2O2 conc, duplicates/triplicates, etc. It also reacts with different pH. If I were you, I would be extra careful about the time of prep and assay, go over the non-diffusible derivates, as well as other options. Good luck.
DCF is diffusible from the cell, so if the preparation and assay take variable time, it will create inconsistent results among H2O2 conc, duplicates/triplicates, etc. It also reacts with different pH. If I were you, I would be extra careful about the time of prep and assay, go over the non-diffusible derivates, as well as other options. Good luck.
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DCF is diffusible from the cell, so if the preparation and assay take variable time, it will create inconsistent results among H2O2 conc, duplicates/triplicates, etc. It also reacts with different pH. If I were you, I would be extra careful about the time of prep and assay, go over the non-diffusible derivates, as well as other options. Good luck.
Article Troubleshooting the dichlorofluorescein assay to avoid artif...
https://www.thermofisher.com/document-connect/document-connect.html?url=https%3A%2F%2Fassets.thermofisher.com%2FTFS-Assets%2FLSG%2Fmanuals%2Fmp36103.pdf
DCF is diffusible from the cell, so if the preparation and assay take variable time, it will create inconsistent results among H2O2 conc, duplicates/triplicates, etc. It also reacts with different pH. If I were you, I would be extra careful about the time of prep and assay, go over the non-diffusible derivates, as well as other options. Good luck.
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Thank you for the answer, I will try your suggestions Shin Murakami
Thank you for the answer, I will try your suggestions Shin Murakami
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