Dear Freja Why did you think the impurities were due to salt? It is not possible to give an answer to your question without knowing the details of the analysis method apllied.
Dear Freja Why did you think the impurities were due to salt? It is not possible to give an answer to your question without knowing the details of the analysis method apllied.
I have looked at the different Bio-spin columns from Biorad and it seems there are several types. Which one can you specifically recommend for purification of amino acids prior to HPLC analysis?
I have looked at the different Bio-spin columns from Biorad and it seems there are several types. Which one can you specifically recommend for purification of amino acids prior to HPLC analysis?
Freja Karlsen BioRad spin columns are size exclusion, so your amino acids should be removed with salts, due to the size. Maybe you can consider by affinity. https://www.thermofisher.com/order/catalog/product/89879
Or you can ask directly to Amos Heckendorf sales@nestgrp.biz from NestGroup https://www.nestgrp.com/protocols/protocol.shtml#deterg
He is very helpful!
Freja Karlsen BioRad spin columns are size exclusion, so your amino acids should be removed with salts, due to the size. Maybe you can consider by affinity. https://www.thermofisher.com/order/catalog/product/89879
Or you can ask directly to Amos Heckendorf sales@nestgrp.biz from NestGroup https://www.nestgrp.com/protocols/protocol.shtml#deterg
He is very helpful!
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Dear Freja
Why did you think the impurities were due to salt? It is not possible to give an answer to your question without knowing the details of the analysis method apllied.
Dear Freja
Why did you think the impurities were due to salt? It is not possible to give an answer to your question without knowing the details of the analysis method apllied.
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Also you can try liquid-liquid extraction technique to purify the sample.
Also you can try liquid-liquid extraction technique to purify the sample.
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BioSpin columns from BioRad, Amicon filter from Merck Millipore or HiPPR™ Detergent Removal Spin Column Kit
BioSpin columns from BioRad, Amicon filter from Merck Millipore or HiPPR™ Detergent Removal Spin Column Kit
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Thaís Pedrete Thank you. It is very helpful.
Thaís Pedrete Thank you. It is very helpful.
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Thaís Pedrete thanks for the recommendations.
I have looked at the different Bio-spin columns from Biorad and it seems there are several types. Which one can you specifically recommend for purification of amino acids prior to HPLC analysis?
Thaís Pedrete thanks for the recommendations.
I have looked at the different Bio-spin columns from Biorad and it seems there are several types. Which one can you specifically recommend for purification of amino acids prior to HPLC analysis?
More
VOTE