Jurkat cels can be activated by exposure to a combination of anti-CD3/anti-CD28 or phytoheamaggutinin (PHA), or the C305 mAb originally developped by Arthur Weiss. IL-2 production should be measured 6 to 12 h later (whatever works best for you). 0.5 to 2 millions cells per ml works. We have used the Jurkat E6.1 variant in our assays. I hope this works for you. Good luck. gilles.dupuis@usherbrooke.ca
Jurkat cels can be activated by exposure to a combination of anti-CD3/anti-CD28 or phytoheamaggutinin (PHA), or the C305 mAb originally developped by Arthur Weiss. IL-2 production should be measured 6 to 12 h later (whatever works best for you). 0.5 to 2 millions cells per ml works. We have used the Jurkat E6.1 variant in our assays. I hope this works for you. Good luck. gilles.dupuis@usherbrooke.ca
Thank you very much, really appreciated.
Thank you very much, really appreciated.
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Jurkat cels can be activated by exposure to a combination of anti-CD3/anti-CD28 or phytoheamaggutinin (PHA), or the C305 mAb originally developped by Arthur Weiss.
IL-2 production should be measured 6 to 12 h later (whatever works best for you).
0.5 to 2 millions cells per ml works. We have used the Jurkat E6.1 variant in our assays.
I hope this works for you.
Good luck.
gilles.dupuis@usherbrooke.ca
Jurkat cels can be activated by exposure to a combination of anti-CD3/anti-CD28 or phytoheamaggutinin (PHA), or the C305 mAb originally developped by Arthur Weiss.
IL-2 production should be measured 6 to 12 h later (whatever works best for you).
0.5 to 2 millions cells per ml works. We have used the Jurkat E6.1 variant in our assays.
I hope this works for you.
Good luck.
gilles.dupuis@usherbrooke.ca
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Hi
Anti-cd3/cd28 Ab and PHA activate Jurkat T cells and induced IL-2 production. Also, you can refer to the following article: 10.1172/JCI112464
Hi
Anti-cd3/cd28 Ab and PHA activate Jurkat T cells and induced IL-2 production. Also, you can refer to the following article: 10.1172/JCI112464
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Hi
Look method attached PLZ
Good luck
Hi
Look method attached PLZ
Good luck
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Hi
Look method attached PLZ
Good luck
Hi
Look method attached PLZ
Good luck
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how do i activate jurkat cells for transendothelial migration?
how do i activate jurkat cells for transendothelial migration?
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