The temperature of EDC/NHS experiment

My experience is at room temperature also - and usually in water.  Can you use a different coupling agent in DMF?

I've always done it at room temperature overnight. I feel like the only time I've seen raised temps for it was to better solvate one of the reagents.

How did it go at 85°C?

Do it in acetonitrile. The DCU byproduct is poorly soluble in it, so the workup is easier.