I do not know which SEM you will be using. For a FESEM, as your sample is non-conducting, I feel that 20kV is very high. Without proper coating, lots of charging will happen at sample and it may even damage the sample. If you are using FESEM, I will suggest proper coating of a conducting material (Au coating) and then start with low voltage such as 3 or 5 kV and try to analyze your sample. Hope this helps.
I do not know which SEM you will be using. For a FESEM, as your sample is non-conducting, I feel that 20kV is very high. Without proper coating, lots of charging will happen at sample and it may even damage the sample. If you are using FESEM, I will suggest proper coating of a conducting material (Au coating) and then start with low voltage such as 3 or 5 kV and try to analyze your sample. Hope this helps.
If your SEM is not too old (20+ years) 20 kV is too high for any observation. Use lower kV, much lower - not only to preserve you specimen, but to get better pictures of any specimen. Observation of gelatin powder at low mags (less than 1000) should not be a problem (it should be coated). At higher mags it could be damaged, but you should try and see how hight in magnification you can go.
If your SEM is not too old (20+ years) 20 kV is too high for any observation. Use lower kV, much lower - not only to preserve you specimen, but to get better pictures of any specimen. Observation of gelatin powder at low mags (less than 1000) should not be a problem (it should be coated). At higher mags it could be damaged, but you should try and see how hight in magnification you can go.
I aggree with the others before. Your sample will charge up quickly at 20KV. Also you will have to sputter Au or other metallic material on your powder to use an SEM anyway. The only exception would be an SEM capable of working under low pressure instead of vakuum. Then your non conductive sample might not load up so quickly, but in this case 20KV also seem to be very much to me.
I aggree with the others before. Your sample will charge up quickly at 20KV. Also you will have to sputter Au or other metallic material on your powder to use an SEM anyway. The only exception would be an SEM capable of working under low pressure instead of vakuum. Then your non conductive sample might not load up so quickly, but in this case 20KV also seem to be very much to me.
I would hardly assume this. But it also depends on the entire energy of the beam, i.e. how big is the beam current. Therefore, watch whether the sample changes during imaging and slowly adapt the conditions. Gelatin often contains water which might affect the imaging conditions as well.
I would hardly assume this. But it also depends on the entire energy of the beam, i.e. how big is the beam current. Therefore, watch whether the sample changes during imaging and slowly adapt the conditions. Gelatin often contains water which might affect the imaging conditions as well.
E-SEM can be an option
E-SEM can be an option
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I do not know which SEM you will be using. For a FESEM, as your sample is non-conducting, I feel that 20kV is very high. Without proper coating, lots of charging will happen at sample and it may even damage the sample. If you are using FESEM, I will suggest proper coating of a conducting material (Au coating) and then start with low voltage such as 3 or 5 kV and try to analyze your sample.
Hope this helps.
I do not know which SEM you will be using. For a FESEM, as your sample is non-conducting, I feel that 20kV is very high. Without proper coating, lots of charging will happen at sample and it may even damage the sample. If you are using FESEM, I will suggest proper coating of a conducting material (Au coating) and then start with low voltage such as 3 or 5 kV and try to analyze your sample.
Hope this helps.
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If your SEM is not too old (20+ years) 20 kV is too high for any observation. Use lower kV, much lower - not only to preserve you specimen, but to get better pictures of any specimen.
Observation of gelatin powder at low mags (less than 1000) should not be a problem (it should be coated). At higher mags it could be damaged, but you should try and see how hight in magnification you can go.
If your SEM is not too old (20+ years) 20 kV is too high for any observation. Use lower kV, much lower - not only to preserve you specimen, but to get better pictures of any specimen.
Observation of gelatin powder at low mags (less than 1000) should not be a problem (it should be coated). At higher mags it could be damaged, but you should try and see how hight in magnification you can go.
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VOTE
I aggree with the others before. Your sample will charge up quickly at 20KV. Also you will have to sputter Au or other metallic material on your powder to use an SEM anyway. The only exception would be an SEM capable of working under low pressure instead of vakuum. Then your non conductive sample might not load up so quickly, but in this case 20KV also seem to be very much to me.
I aggree with the others before. Your sample will charge up quickly at 20KV. Also you will have to sputter Au or other metallic material on your powder to use an SEM anyway. The only exception would be an SEM capable of working under low pressure instead of vakuum. Then your non conductive sample might not load up so quickly, but in this case 20KV also seem to be very much to me.
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i2oKV is high value
i2oKV is high value
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I would hardly assume this. But it also depends on the entire energy of the beam, i.e. how big is the beam current. Therefore, watch whether the sample changes during imaging and slowly adapt the conditions. Gelatin often contains water which might affect the imaging conditions as well.
I would hardly assume this. But it also depends on the entire energy of the beam, i.e. how big is the beam current. Therefore, watch whether the sample changes during imaging and slowly adapt the conditions. Gelatin often contains water which might affect the imaging conditions as well.
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i mean to use normal SEM not FESEM
i mean to use normal SEM not FESEM
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