Hi Fuad, we use an amide column run in HILIC mode to separate pyridoxine, riboflavin, ascorbic acid, folic acid and cyanocobalamin. The resolution is very good. Pyridoxine and especially folic acid resolve into multiple peaks.
Hi Fuad, we use an amide column run in HILIC mode to separate pyridoxine, riboflavin, ascorbic acid, folic acid and cyanocobalamin. The resolution is very good. Pyridoxine and especially folic acid resolve into multiple peaks.
for polar analytes, the choice would be any normal phase column. Off course, the efficiency of different NP columns have to be checked for this specific problem
for polar analytes, the choice would be any normal phase column. Off course, the efficiency of different NP columns have to be checked for this specific problem
ZIC HILIC from Merck Millipore is the best option.visit www.merckmillipore.com/chromatography and click Application finder. Apart from this Amino columns can be a better option for some vitamins.
ZIC HILIC from Merck Millipore is the best option.visit www.merckmillipore.com/chromatography and click Application finder. Apart from this Amino columns can be a better option for some vitamins.
In general for polar water-soluble vitamins you want to use a reversed-phase (RP) column that can tolerate high aqueous content (even 100% aqueous) as well as acidic conditions. These columns are usually either polar-embedded RP columns or hyperbranched RP columns. The following link shows work in my lab by Dr. Deanna Hurum with a hyperbranched style column. Using either style of column lets you avoid ion-pairing reagents. http://www.dionex.com/en-us/webdocs/71938-AN216-HPLC-Vitamins-FunctionalWaters-30April09-LPN2145.pdf
In general for polar water-soluble vitamins you want to use a reversed-phase (RP) column that can tolerate high aqueous content (even 100% aqueous) as well as acidic conditions. These columns are usually either polar-embedded RP columns or hyperbranched RP columns. The following link shows work in my lab by Dr. Deanna Hurum with a hyperbranched style column. Using either style of column lets you avoid ion-pairing reagents. http://www.dionex.com/en-us/webdocs/71938-AN216-HPLC-Vitamins-FunctionalWaters-30April09-LPN2145.pdf
Hi Fuad, Perhaps I can help bridge the gap between the two options presented here. I did a bit of research, and found water-soluble vitamins can be run on both HILIC and Reverse Phase C18!
In the case against Si(2) and HILIC, that's an easy answer... HILIC all the way. About six or seven years ago, we used to sell Si(2) columns ALL the time. Now a days, people only buy HILIC... because it is very reproducible, and you don't have the water pre-saturation problem you have with Si(2)
For your convenience, I have also provided a couple of applications for you. Does this answer your question? Paul
Hi Fuad, Perhaps I can help bridge the gap between the two options presented here. I did a bit of research, and found water-soluble vitamins can be run on both HILIC and Reverse Phase C18!
In the case against Si(2) and HILIC, that's an easy answer... HILIC all the way. About six or seven years ago, we used to sell Si(2) columns ALL the time. Now a days, people only buy HILIC... because it is very reproducible, and you don't have the water pre-saturation problem you have with Si(2)
For your convenience, I have also provided a couple of applications for you. Does this answer your question? Paul
I'm totaly agree with Mr Paul Karch. It also depends of the investissement amount you have to perform your analysis (and what the versatility of the presume column after your separation)
I'm totaly agree with Mr Paul Karch. It also depends of the investissement amount you have to perform your analysis (and what the versatility of the presume column after your separation)
Here is the other file...
Here is the other file...
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Rp c18
reverse fase
Rp c18
reverse fase
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Hi Fuad, we use an amide column run in HILIC mode to separate pyridoxine, riboflavin, ascorbic acid, folic acid and cyanocobalamin. The resolution is very good. Pyridoxine and especially folic acid resolve into multiple peaks.
Hi Fuad, we use an amide column run in HILIC mode to separate pyridoxine, riboflavin, ascorbic acid, folic acid and cyanocobalamin. The resolution is very good. Pyridoxine and especially folic acid resolve into multiple peaks.
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for polar analytes, the choice would be any normal phase column. Off course, the efficiency of different NP columns have to be checked for this specific problem
for polar analytes, the choice would be any normal phase column. Off course, the efficiency of different NP columns have to be checked for this specific problem
More
VOTE
ZIC HILIC from Merck Millipore is the best option.visit www.merckmillipore.com/chromatography and click Application finder.
Apart from this Amino columns can be a better option for some vitamins.
ZIC HILIC from Merck Millipore is the best option.visit www.merckmillipore.com/chromatography and click Application finder.
Apart from this Amino columns can be a better option for some vitamins.
More
VOTE
In general for polar water-soluble vitamins you want to use a reversed-phase (RP) column that can tolerate high aqueous content (even 100% aqueous) as well as acidic conditions. These columns are usually either polar-embedded RP columns or hyperbranched RP columns. The following link shows work in my lab by Dr. Deanna Hurum with a hyperbranched style column. Using either style of column lets you avoid ion-pairing reagents.
http://www.dionex.com/en-us/webdocs/71938-AN216-HPLC-Vitamins-FunctionalWaters-30April09-LPN2145.pdf
In general for polar water-soluble vitamins you want to use a reversed-phase (RP) column that can tolerate high aqueous content (even 100% aqueous) as well as acidic conditions. These columns are usually either polar-embedded RP columns or hyperbranched RP columns. The following link shows work in my lab by Dr. Deanna Hurum with a hyperbranched style column. Using either style of column lets you avoid ion-pairing reagents.
http://www.dionex.com/en-us/webdocs/71938-AN216-HPLC-Vitamins-FunctionalWaters-30April09-LPN2145.pdf
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I use a method with C18 that can separate until 9 vitamins in 15 minutes
I use a method with C18 that can separate until 9 vitamins in 15 minutes
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I use RP c18 for analise viatmins B group.
I use RP c18 for analise viatmins B group.
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Hi Fuad,
Perhaps I can help bridge the gap between the two options presented here. I did a bit of research, and found water-soluble vitamins can be run on both HILIC and Reverse Phase C18!
In the case against Si(2) and HILIC, that's an easy answer... HILIC all the way. About six or seven years ago, we used to sell Si(2) columns ALL the time. Now a days, people only buy HILIC... because it is very reproducible, and you don't have the water pre-saturation problem you have with Si(2)
For your convenience, I have also provided a couple of applications for you. Does this answer your question?
Paul
Hi Fuad,
Perhaps I can help bridge the gap between the two options presented here. I did a bit of research, and found water-soluble vitamins can be run on both HILIC and Reverse Phase C18!
In the case against Si(2) and HILIC, that's an easy answer... HILIC all the way. About six or seven years ago, we used to sell Si(2) columns ALL the time. Now a days, people only buy HILIC... because it is very reproducible, and you don't have the water pre-saturation problem you have with Si(2)
For your convenience, I have also provided a couple of applications for you. Does this answer your question?
Paul
More
VOTE
I'm totaly agree with Mr Paul Karch. It also depends of the investissement amount you have to perform your analysis (and what the versatility of the presume column after your separation)
I'm totaly agree with Mr Paul Karch. It also depends of the investissement amount you have to perform your analysis (and what the versatility of the presume column after your separation)
More
VOTE