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What is the principle of vanillin-sulfuric acid assay to quantify...
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+ Banana
+ Saponins
+ Peeling
+ Sulfuric acids
+ Extraction
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Mel B.
What is the principle of vanillin-sulfuric acid assay to quantify...
Hi Afrina Zainal, It was likely the solvent you used to extract saponins that caused the darkening of the reaction mixture after incubation. If it was the case, this article would help.
Hi Afrina Zainal, It was likely the solvent you used to extract saponins that caused the darkening of the reaction mixture after incubation. If it was the case, this article would help.
It's a free radical reaction mechanism which make it complicate to specify what really happens. There is a hole method. But mostly use vanilla acid solutions. Water will be a problem led to low results.
It's a free radical reaction mechanism which make it complicate to specify what really happens. There is a hole method. But mostly use vanilla acid solutions. Water will be a problem led to low results.
Hi Afrina Zainal,
It was likely the solvent you used to extract saponins that caused the darkening of the reaction mixture after incubation. If it was the case, this article would help.
Hi Afrina Zainal,
It was likely the solvent you used to extract saponins that caused the darkening of the reaction mixture after incubation. If it was the case, this article would help.
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for us, we used the method of vanilin perchloric acid
for us, we used the method of vanilin perchloric acid
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In my assays the same thing happens...when I add the acid, the mixture becomes yellow. Did you find a explanation or solution?
Thanks
In my assays the same thing happens...when I add the acid, the mixture becomes yellow. Did you find a explanation or solution?
Thanks
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Thank you Mr. Amit and Mr. Amir, coincidentally both of you share the same article. But not much information I can use from there.
Thank you Mr. Amit and Mr. Amir, coincidentally both of you share the same article. But not much information I can use from there.
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Dear Sabah Mabrouki, may i know what colour of the reaction do u observe? is the procedure similar to what i do?
Dear Sabah Mabrouki, may i know what colour of the reaction do u observe? is the procedure similar to what i do?
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if the colure is changing then you may need to protect from light. but try the link below.
https://www.users.miamioh.edu/hagermae/Vanillin%20Assay.pdf
if the colure is changing then you may need to protect from light. but try the link below.
https://www.users.miamioh.edu/hagermae/Vanillin%20Assay.pdf
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Please find the detailed method of vanillin reaction and its principle in the attached article.
Please find the detailed method of vanillin reaction and its principle in the attached article.
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It's a free radical reaction mechanism which make it complicate to specify what really happens. There is a hole method. But mostly use vanilla acid solutions. Water will be a problem led to low results.
It's a free radical reaction mechanism which make it complicate to specify what really happens. There is a hole method. But mostly use vanilla acid solutions. Water will be a problem led to low results.
More
VOTE