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Any idea why the colored product of ABTS + H2O2 reaction is...
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+ Elisa
+ Reaction kinetics
+ Immunoassay
+ Colorimetry
Posted by
Mineta Harlow
Any idea why the colored product of ABTS + H2O2 reaction is...
I am running an experiment testing HRP-activity of Gquadruplex and hemin. Reaction works and ABTS turns blue and I can read a significant the absorbance change within seconds. But my problem is that the color turns back to yellow quickly. as if ABTS is reduced back to original form. Any ideas why?
