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Are SPME fibres reusable? If yes, how many times?
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+ Volatile organic compounds
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Alan Jia
Are SPME fibres reusable? If yes, how many times?
The way we check the decline of the fiber with a model mixture is very usefull in order to decide when a fiber is too old and needs to be discarded. We have an automatic sampler (Gerstel coupled with GC-MS) and usually with DVB/CARB/PDMS or CARB/PDMS we used 50-60 times for Headspace-SPME determinations of VOC in polymer.
The way we check the decline of the fiber with a model mixture is very usefull in order to decide when a fiber is too old and needs to be discarded. We have an automatic sampler (Gerstel coupled with GC-MS) and usually with DVB/CARB/PDMS or CARB/PDMS we used 50-60 times for Headspace-SPME determinations of VOC in polymer.
Indeed, they are reusable. In our experience can be reused more than 100 times without problems. It is a robust and simple technique for what you want to measure. Check my publication in my profile.
Indeed, they are reusable. In our experience can be reused more than 100 times without problems. It is a robust and simple technique for what you want to measure. Check my publication in my profile.
SPME fibers can be used according to the manufacturer for 100 injections, but their use depends as mentioned before, the analytical matrix , temperature, and extraction mode , if headspace can reach up to 200 shots, but if Immersive much less .
SPME fibers can be used according to the manufacturer for 100 injections, but their use depends as mentioned before, the analytical matrix , temperature, and extraction mode , if headspace can reach up to 200 shots, but if Immersive much less .
Iwould use PA ones. I used for more than 200 injection without loose of activity. Another important thing is if you use acids to lower the pH. It strongly affects the fiber lasting.
Charlie Rich, EV and Renewables FanaticFollowFollowing
Iwould use PA ones. I used for more than 200 injection without loose of activity. Another important thing is if you use acids to lower the pH. It strongly affects the fiber lasting.
Absolutely, they are reusable. How many times will depend on the temperature of your injector, the nature of your analytes, etc. With the appropriate QA/QC protocol you will find out when the retention capability of your fiber starts to decline.
Absolutely, they are reusable. How many times will depend on the temperature of your injector, the nature of your analytes, etc. With the appropriate QA/QC protocol you will find out when the retention capability of your fiber starts to decline.
With no handlig mistakes, for headspace analysis, using a PDMS fiber at least 100 extractions. I got once a PDMS working almost a year, almost all days. Other fibers are not so robust. The DVB/car/PDMS will be the best choive for bacterial volatiles however it wont last as much as a PDMS fiber.
With no handlig mistakes, for headspace analysis, using a PDMS fiber at least 100 extractions. I got once a PDMS working almost a year, almost all days. Other fibers are not so robust. The DVB/car/PDMS will be the best choive for bacterial volatiles however it wont last as much as a PDMS fiber.
The SPME fibers are reusable if you want to analyze headspace volatiles from bacterial cultures by SPME-GC-MS. You could use only one fiber for 50-100 times. However, you have to be careful. The fibers are fragile.
The SPME fibers are reusable if you want to analyze headspace volatiles from bacterial cultures by SPME-GC-MS. You could use only one fiber for 50-100 times. However, you have to be careful. The fibers are fragile.
Yes SPME fibre are reusable but their capacity to sorb the analytes declined with time and number of injections. In order to test this change we make a test along time with a model mixture of known volatile compounds in a non-volatil solvent.
Yes SPME fibre are reusable but their capacity to sorb the analytes declined with time and number of injections. In order to test this change we make a test along time with a model mixture of known volatile compounds in a non-volatil solvent.
With manual use, number of samples per fiber largely depends on analyst care and fatigue. With automated SPME we have experienced up to 1000 samples even with the more sensitive dvb/car/pdms fiber.
With manual use, number of samples per fiber largely depends on analyst care and fatigue. With automated SPME we have experienced up to 1000 samples even with the more sensitive dvb/car/pdms fiber.
The way we check the decline of the fiber with a model mixture is very usefull in order to decide when a fiber is too old and needs to be discarded. We have an automatic sampler (Gerstel coupled with GC-MS) and usually with DVB/CARB/PDMS or CARB/PDMS we used 50-60 times for Headspace-SPME determinations of VOC in polymer.
The way we check the decline of the fiber with a model mixture is very usefull in order to decide when a fiber is too old and needs to be discarded. We have an automatic sampler (Gerstel coupled with GC-MS) and usually with DVB/CARB/PDMS or CARB/PDMS we used 50-60 times for Headspace-SPME determinations of VOC in polymer.
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Indeed, they are reusable. In our experience can be reused more than 100 times without problems. It is a robust and simple technique for what you want to measure. Check my publication in my profile.
Indeed, they are reusable. In our experience can be reused more than 100 times without problems. It is a robust and simple technique for what you want to measure. Check my publication in my profile.
More
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SPME fibers can be used according to the manufacturer for 100 injections, but their use depends as mentioned before, the analytical matrix , temperature, and extraction mode , if headspace can reach up to 200 shots, but if Immersive much less .
SPME fibers can be used according to the manufacturer for 100 injections, but their use depends as mentioned before, the analytical matrix , temperature, and extraction mode , if headspace can reach up to 200 shots, but if Immersive much less .
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Iwould use PA ones. I used for more than 200 injection without loose of activity. Another important thing is if you use acids to lower the pH. It strongly affects the fiber lasting.
Iwould use PA ones. I used for more than 200 injection without loose of activity. Another important thing is if you use acids to lower the pH. It strongly affects the fiber lasting.
More
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Absolutely, they are reusable. How many times will depend on the temperature of your injector, the nature of your analytes, etc. With the appropriate QA/QC protocol you will find out when the retention capability of your fiber starts to decline.
Absolutely, they are reusable. How many times will depend on the temperature of your injector, the nature of your analytes, etc. With the appropriate QA/QC protocol you will find out when the retention capability of your fiber starts to decline.
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With no handlig mistakes, for headspace analysis, using a PDMS fiber at least 100 extractions. I got once a PDMS working almost a year, almost all days. Other fibers are not so robust. The DVB/car/PDMS will be the best choive for bacterial volatiles however it wont last as much as a PDMS fiber.
With no handlig mistakes, for headspace analysis, using a PDMS fiber at least 100 extractions. I got once a PDMS working almost a year, almost all days. Other fibers are not so robust. The DVB/car/PDMS will be the best choive for bacterial volatiles however it wont last as much as a PDMS fiber.
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The SPME fibers are reusable if you want to analyze headspace volatiles from bacterial cultures by SPME-GC-MS. You could use only one fiber for 50-100 times. However, you have to be careful. The fibers are fragile.
The SPME fibers are reusable if you want to analyze headspace volatiles from bacterial cultures by SPME-GC-MS. You could use only one fiber for 50-100 times. However, you have to be careful. The fibers are fragile.
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Yes SPME fibre are reusable but their capacity to sorb the analytes declined with time and number of injections. In order to test this change we make a test along time with a model mixture of known volatile compounds in a non-volatil solvent.
Yes SPME fibre are reusable but their capacity to sorb the analytes declined with time and number of injections. In order to test this change we make a test along time with a model mixture of known volatile compounds in a non-volatil solvent.
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lt's better to dilute your culture samples before Head Space SPME procedure for long-term fiber using.
lt's better to dilute your culture samples before Head Space SPME procedure for long-term fiber using.
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I use it several times between 100-120
I use it several times between 100-120
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With manual use, number of samples per fiber largely depends on analyst care and fatigue. With automated SPME we have experienced up to 1000 samples even with the more sensitive dvb/car/pdms fiber.
With manual use, number of samples per fiber largely depends on analyst care and fatigue. With automated SPME we have experienced up to 1000 samples even with the more sensitive dvb/car/pdms fiber.
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