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Can any one explain how to determine diastereomeric ratio from NMR...
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+ Stereochemistry
+ Nuclear magnetic resonance
+ Nmr spectroscopy
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Laurence Cuffe
Can any one explain how to determine diastereomeric ratio from NMR...
Select a pair of suitable signals (one from each diastereomer) and do a careful integration. This gives good results only when the nuclei are fully relaxed. It is therefore best to use 1H NMR signals. 13C usually relaxes more slowly and suffers from incorrect signal intensities due to nuclear Overhauser effects as a consequence of proton decoupling.
Select a pair of suitable signals (one from each diastereomer) and do a careful integration. This gives good results only when the nuclei are fully relaxed. It is therefore best to use 1H NMR signals. 13C usually relaxes more slowly and suffers from incorrect signal intensities due to nuclear Overhauser effects as a consequence of proton decoupling.
Select a pair of suitable signals (one from each diastereomer) and do a careful integration. This gives good results only when the nuclei are fully relaxed. It is therefore best to use 1H NMR signals. 13C usually relaxes more slowly and suffers from incorrect signal intensities due to nuclear Overhauser effects as a consequence of proton decoupling.
Select a pair of suitable signals (one from each diastereomer) and do a careful integration. This gives good results only when the nuclei are fully relaxed. It is therefore best to use 1H NMR signals. 13C usually relaxes more slowly and suffers from incorrect signal intensities due to nuclear Overhauser effects as a consequence of proton decoupling.
As Prof. Ernst stated, you first need to make sure to choose a correct pair of equivalent but not stereochemically equal 1H NMR signals (due to fast relaxation times), preferably of methylene or methyl groups and also preferably of low coupling patterns. Each one of them belonging to one of the two subjected diastereoisomers and thus integrate them to obtain the desired diastereomeric ratio proportion. Make sure with COSY and HETCOR (HSQC) 2D NMR experiments that they are unique signals to avoid missintegration. Best regards and experiments. Hiram
As Prof. Ernst stated, you first need to make sure to choose a correct pair of equivalent but not stereochemically equal 1H NMR signals (due to fast relaxation times), preferably of methylene or methyl groups and also preferably of low coupling patterns. Each one of them belonging to one of the two subjected diastereoisomers and thus integrate them to obtain the desired diastereomeric ratio proportion. Make sure with COSY and HETCOR (HSQC) 2D NMR experiments that they are unique signals to avoid missintegration. Best regards and experiments. Hiram
Select a pair of suitable signals (one from each diastereomer) and do a careful integration. This gives good results only when the nuclei are fully relaxed. It is therefore best to use 1H NMR signals. 13C usually relaxes more slowly and suffers from incorrect signal intensities due to nuclear Overhauser effects as a consequence of proton decoupling.
Select a pair of suitable signals (one from each diastereomer) and do a careful integration. This gives good results only when the nuclei are fully relaxed. It is therefore best to use 1H NMR signals. 13C usually relaxes more slowly and suffers from incorrect signal intensities due to nuclear Overhauser effects as a consequence of proton decoupling.
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Please check the link below ( in Chem. Commun., 2014,50, 2512-2514) and I hope to be useful for you
http://pubs.rsc.org/en/Content/ArticleLanding/2014/CC/c3cc49659g#!divAbstract
Please check the link below ( in Chem. Commun., 2014,50, 2512-2514) and I hope to be useful for you
http://pubs.rsc.org/en/Content/ArticleLanding/2014/CC/c3cc49659g#!divAbstract
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http://en.wikipedia.org/wiki/NMR_spectroscopy_of_stereoisomers
http://en.wikipedia.org/wiki/NMR_spectroscopy_of_stereoisomers
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Further to what has been stated earlier, please make sure you do baseline correction of your spectrum.
Further to what has been stated earlier, please make sure you do baseline correction of your spectrum.
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Select a pair of suitable signals (one from each diastereomer) and do a careful integration. This gives good results only when the nuclei are fully relaxed. It is therefore best to use 1H NMR signals. 13C usually relaxes more slowly and suffers from incorrect signal intensities due to nuclear Overhauser effects as a consequence of proton decoupling.
Select a pair of suitable signals (one from each diastereomer) and do a careful integration. This gives good results only when the nuclei are fully relaxed. It is therefore best to use 1H NMR signals. 13C usually relaxes more slowly and suffers from incorrect signal intensities due to nuclear Overhauser effects as a consequence of proton decoupling.
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As Prof. Ernst stated, you first need to make sure to choose a correct pair of equivalent but not stereochemically equal 1H NMR signals (due to fast relaxation times), preferably of methylene or methyl groups and also preferably of low coupling patterns. Each one of them belonging to one of the two subjected diastereoisomers and thus integrate them to obtain the desired diastereomeric ratio proportion. Make sure with COSY and HETCOR (HSQC) 2D NMR experiments that they are unique signals to avoid missintegration. Best regards and experiments. Hiram
As Prof. Ernst stated, you first need to make sure to choose a correct pair of equivalent but not stereochemically equal 1H NMR signals (due to fast relaxation times), preferably of methylene or methyl groups and also preferably of low coupling patterns. Each one of them belonging to one of the two subjected diastereoisomers and thus integrate them to obtain the desired diastereomeric ratio proportion. Make sure with COSY and HETCOR (HSQC) 2D NMR experiments that they are unique signals to avoid missintegration. Best regards and experiments. Hiram
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