Home >
Community >
Can biotinylated primers pull down long, circular DNA?
Upvote
28
Downvote
+ Plasmids
+ Biotinylation
+ Primer
Posted by
Max Winkler
Can biotinylated primers pull down long, circular DNA?
Covalently closed circular DNA renatures readily once denaturing conditions are removed, either by cooling (heat denaturation) or neutralization (alkali denaturation); this is the principle of plasmid isolation. To give your biotinylated primers a chance to hybridize, you should add them together with the plasmid before proceeding to the denaturation-renaturation step
Covalently closed circular DNA renatures readily once denaturing conditions are removed, either by cooling (heat denaturation) or neutralization (alkali denaturation); this is the principle of plasmid isolation. To give your biotinylated primers a chance to hybridize, you should add them together with the plasmid before proceeding to the denaturation-renaturation step
Covalently closed circular DNA renatures readily once denaturing conditions are removed, either by cooling (heat denaturation) or neutralization (alkali denaturation); this is the principle of plasmid isolation. To give your biotinylated primers a chance to hybridize, you should add them together with the plasmid before proceeding to the denaturation-renaturation step
Covalently closed circular DNA renatures readily once denaturing conditions are removed, either by cooling (heat denaturation) or neutralization (alkali denaturation); this is the principle of plasmid isolation. To give your biotinylated primers a chance to hybridize, you should add them together with the plasmid before proceeding to the denaturation-renaturation step
More
VOTE