To strictly answer the question its not possible because it is a pipeline based on Primer3 which is not written by NCBI, therefore they would not be in a position to distribute it. Nor would Primer3 be able to distribute Blast because that is NCBI. It is simply a web-server.
NCBI and Primer3 would need to come to a joint agreement, which they must have done to present the webserver (at least it would be polite). You can of course replicate the pipeline yourself.
Wait it can be done via Docker. This exact pipeline hasn't been distributed via Docker but others have and Blast is available as a Docker image. So I correct myself ... its definitely feasible. Using Docker takes a bit of a learning curve but its worth it.
To strictly answer the question its not possible because it is a pipeline based on Primer3 which is not written by NCBI, therefore they would not be in a position to distribute it. Nor would Primer3 be able to distribute Blast because that is NCBI. It is simply a web-server.
NCBI and Primer3 would need to come to a joint agreement, which they must have done to present the webserver (at least it would be polite). You can of course replicate the pipeline yourself.
Wait it can be done via Docker. This exact pipeline hasn't been distributed via Docker but others have and Blast is available as a Docker image. So I correct myself ... its definitely feasible. Using Docker takes a bit of a learning curve but its worth it.
To strictly answer the question its not possible because it is a pipeline based on Primer3 which is not written by NCBI, therefore they would not be in a position to distribute it. Nor would Primer3 be able to distribute Blast because that is NCBI. It is simply a web-server.
NCBI and Primer3 would need to come to a joint agreement, which they must have done to present the webserver (at least it would be polite). You can of course replicate the pipeline yourself.
Brief details are here, https://www.ncbi.nlm.nih.gov/tools/primer-blast/primerinfo.html
Wait it can be done via Docker. This exact pipeline hasn't been distributed via Docker but others have and Blast is available as a Docker image. So I correct myself ... its definitely feasible. Using Docker takes a bit of a learning curve but its worth it.
To strictly answer the question its not possible because it is a pipeline based on Primer3 which is not written by NCBI, therefore they would not be in a position to distribute it. Nor would Primer3 be able to distribute Blast because that is NCBI. It is simply a web-server.
NCBI and Primer3 would need to come to a joint agreement, which they must have done to present the webserver (at least it would be polite). You can of course replicate the pipeline yourself.
Brief details are here,https://www.ncbi.nlm.nih.gov/tools/primer-blast/primerinfo.html
Wait it can be done via Docker. This exact pipeline hasn't been distributed via Docker but others have and Blast is available as a Docker image. So I correct myself ... its definitely feasible. Using Docker takes a bit of a learning curve but its worth it.
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