You may prepare the stock of PMA in DMSO (1mg PMA in 1ml DMSO). (1mg/ml) You may aliquot the stock PMA and freeze.
When you are preparing working solutions such as 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml, and 50ng/ml diluted in culture medium, they have to be prepared fresh on the day of the experiment. Do not prepare working solutions and freeze before the experiment.
On the day of the experiment, take one vial of 1ul stock PMA (1mg/ml), and dilute the stock PMA (1:1000) with culture medium to get a diluted stock of 1ug/ml (i.e. 1ul of stock PMA + 999ul of culture medium).
To prepare 2.5ng/ml working solution, you should take 2.5ul of the above diluted stock (1ug/ml) and add to 997.5ul of culture medium to make the volume of the working solution to 1ml. Similarly, For 5ng/ml: Take 5ul of diluted stock (1ug/ml) and add to 995ul of culture medium to make the volume of the working solution to 1ml. For 10ng/ml: Take 10ul of diluted stock (1ug/ml) and add to 990ul of culture medium to make the volume of the working solution to 1ml. For 20ng/ml: Take 20ul of diluted stock (1ug/ml) and add to 980ul of culture medium to make the volume of the working solution to 1ml. For 50ng/ml: Take 50ul of diluted stock (1ug/ml) and add to 950ul of culture medium to make the volume of the working solution to 1ml. Use these working solutions for the experiment on the same day.
You may prepare the stock of PMA in DMSO (1mg PMA in 1ml DMSO). (1mg/ml) You may aliquot the stock PMA and freeze.
When you are preparing working solutions such as 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml, and 50ng/ml diluted in culture medium, they have to be prepared fresh on the day of the experiment. Do not prepare working solutions and freeze before the experiment.
On the day of the experiment, take one vial of 1ul stock PMA (1mg/ml), and dilute the stock PMA (1:1000) with culture medium to get a diluted stock of 1ug/ml (i.e. 1ul of stock PMA + 999ul of culture medium).
To prepare 2.5ng/ml working solution, you should take 2.5ul of the above diluted stock (1ug/ml) and add to 997.5ul of culture medium to make the volume of the working solution to 1ml. Similarly, For 5ng/ml: Take 5ul of diluted stock (1ug/ml) and add to 995ul of culture medium to make the volume of the working solution to 1ml. For 10ng/ml: Take 10ul of diluted stock (1ug/ml) and add to 990ul of culture medium to make the volume of the working solution to 1ml. For 20ng/ml: Take 20ul of diluted stock (1ug/ml) and add to 980ul of culture medium to make the volume of the working solution to 1ml. For 50ng/ml: Take 50ul of diluted stock (1ug/ml) and add to 950ul of culture medium to make the volume of the working solution to 1ml. Use these working solutions for the experiment on the same day.
You may prepare the stock of PMA in DMSO (1mg PMA in 1ml DMSO). (1mg/ml) You may aliquot the stock PMA and freeze.
When you are preparing working solutions such as 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml, and 50ng/ml diluted in culture medium, they have to be prepared fresh on the day of the experiment. Do not prepare working solutions and freeze before the experiment.
On the day of the experiment, take one vial of 1ul stock PMA (1mg/ml), and dilute the stock PMA (1:1000) with culture medium to get a diluted stock of 1ug/ml (i.e. 1ul of stock PMA + 999ul of culture medium).
To prepare 2.5ng/ml working solution, you should take 2.5ul of the above diluted stock (1ug/ml) and add to 997.5ul of culture medium to make the volume of the working solution to 1ml. Similarly, For 5ng/ml: Take 5ul of diluted stock (1ug/ml) and add to 995ul of culture medium to make the volume of the working solution to 1ml. For 10ng/ml: Take 10ul of diluted stock (1ug/ml) and add to 990ul of culture medium to make the volume of the working solution to 1ml. For 20ng/ml: Take 20ul of diluted stock (1ug/ml) and add to 980ul of culture medium to make the volume of the working solution to 1ml. For 50ng/ml: Take 50ul of diluted stock (1ug/ml) and add to 950ul of culture medium to make the volume of the working solution to 1ml. Use these working solutions for the experiment on the same day.
You may prepare the stock of PMA in DMSO (1mg PMA in 1ml DMSO). (1mg/ml) You may aliquot the stock PMA and freeze.
When you are preparing working solutions such as 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml, and 50ng/ml diluted in culture medium, they have to be prepared fresh on the day of the experiment. Do not prepare working solutions and freeze before the experiment.
On the day of the experiment, take one vial of 1ul stock PMA (1mg/ml), and dilute the stock PMA (1:1000) with culture medium to get a diluted stock of 1ug/ml (i.e. 1ul of stock PMA + 999ul of culture medium).
To prepare 2.5ng/ml working solution, you should take 2.5ul of the above diluted stock (1ug/ml) and add to 997.5ul of culture medium to make the volume of the working solution to 1ml. Similarly, For 5ng/ml: Take 5ul of diluted stock (1ug/ml) and add to 995ul of culture medium to make the volume of the working solution to 1ml. For 10ng/ml: Take 10ul of diluted stock (1ug/ml) and add to 990ul of culture medium to make the volume of the working solution to 1ml. For 20ng/ml: Take 20ul of diluted stock (1ug/ml) and add to 980ul of culture medium to make the volume of the working solution to 1ml. For 50ng/ml: Take 50ul of diluted stock (1ug/ml) and add to 950ul of culture medium to make the volume of the working solution to 1ml. Use these working solutions for the experiment on the same day.
Dear Louise Dolan
You may prepare the stock of PMA in DMSO (1mg PMA in 1ml DMSO). (1mg/ml)
You may aliquot the stock PMA and freeze.
When you are preparing working solutions such as 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml, and 50ng/ml diluted in culture medium, they have to be prepared fresh on the day of the experiment. Do not prepare working solutions and freeze before the experiment.
On the day of the experiment, take one vial of 1ul stock PMA (1mg/ml), and dilute the stock PMA (1:1000) with culture medium to get a diluted stock of 1ug/ml (i.e. 1ul of stock PMA + 999ul of culture medium).
To prepare 2.5ng/ml working solution, you should take 2.5ul of the above diluted stock (1ug/ml) and add to 997.5ul of culture medium to make the volume of the working solution to 1ml.
Similarly,
For 5ng/ml: Take 5ul of diluted stock (1ug/ml) and add to 995ul of culture medium to make the volume of the working solution to 1ml.
For 10ng/ml: Take 10ul of diluted stock (1ug/ml) and add to 990ul of culture medium to make the volume of the working solution to 1ml.
For 20ng/ml: Take 20ul of diluted stock (1ug/ml) and add to 980ul of culture medium to make the volume of the working solution to 1ml.
For 50ng/ml: Take 50ul of diluted stock (1ug/ml) and add to 950ul of culture medium to make the volume of the working solution to 1ml.
Use these working solutions for the experiment on the same day.
Good Luck.
Dear Louise Dolan
You may prepare the stock of PMA in DMSO (1mg PMA in 1ml DMSO). (1mg/ml)
You may aliquot the stock PMA and freeze.
When you are preparing working solutions such as 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml, and 50ng/ml diluted in culture medium, they have to be prepared fresh on the day of the experiment. Do not prepare working solutions and freeze before the experiment.
On the day of the experiment, take one vial of 1ul stock PMA (1mg/ml), and dilute the stock PMA (1:1000) with culture medium to get a diluted stock of 1ug/ml (i.e. 1ul of stock PMA + 999ul of culture medium).
To prepare 2.5ng/ml working solution, you should take 2.5ul of the above diluted stock (1ug/ml) and add to 997.5ul of culture medium to make the volume of the working solution to 1ml.
Similarly,
For 5ng/ml: Take 5ul of diluted stock (1ug/ml) and add to 995ul of culture medium to make the volume of the working solution to 1ml.
For 10ng/ml: Take 10ul of diluted stock (1ug/ml) and add to 990ul of culture medium to make the volume of the working solution to 1ml.
For 20ng/ml: Take 20ul of diluted stock (1ug/ml) and add to 980ul of culture medium to make the volume of the working solution to 1ml.
For 50ng/ml: Take 50ul of diluted stock (1ug/ml) and add to 950ul of culture medium to make the volume of the working solution to 1ml.
Use these working solutions for the experiment on the same day.
Good Luck.
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Dear Louise Dolan
You may prepare the stock of PMA in DMSO (1mg PMA in 1ml DMSO). (1mg/ml)
You may aliquot the stock PMA and freeze.
When you are preparing working solutions such as 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml, and 50ng/ml diluted in culture medium, they have to be prepared fresh on the day of the experiment. Do not prepare working solutions and freeze before the experiment.
On the day of the experiment, take one vial of 1ul stock PMA (1mg/ml), and dilute the stock PMA (1:1000) with culture medium to get a diluted stock of 1ug/ml (i.e. 1ul of stock PMA + 999ul of culture medium).
To prepare 2.5ng/ml working solution, you should take 2.5ul of the above diluted stock (1ug/ml) and add to 997.5ul of culture medium to make the volume of the working solution to 1ml.
Similarly,
For 5ng/ml: Take 5ul of diluted stock (1ug/ml) and add to 995ul of culture medium to make the volume of the working solution to 1ml.
For 10ng/ml: Take 10ul of diluted stock (1ug/ml) and add to 990ul of culture medium to make the volume of the working solution to 1ml.
For 20ng/ml: Take 20ul of diluted stock (1ug/ml) and add to 980ul of culture medium to make the volume of the working solution to 1ml.
For 50ng/ml: Take 50ul of diluted stock (1ug/ml) and add to 950ul of culture medium to make the volume of the working solution to 1ml.
Use these working solutions for the experiment on the same day.
Good Luck.
Dear Louise Dolan
You may prepare the stock of PMA in DMSO (1mg PMA in 1ml DMSO). (1mg/ml)
You may aliquot the stock PMA and freeze.
When you are preparing working solutions such as 2.5ng/ml, 5ng/ml, 10ng/ml, 20ng/ml, and 50ng/ml diluted in culture medium, they have to be prepared fresh on the day of the experiment. Do not prepare working solutions and freeze before the experiment.
On the day of the experiment, take one vial of 1ul stock PMA (1mg/ml), and dilute the stock PMA (1:1000) with culture medium to get a diluted stock of 1ug/ml (i.e. 1ul of stock PMA + 999ul of culture medium).
To prepare 2.5ng/ml working solution, you should take 2.5ul of the above diluted stock (1ug/ml) and add to 997.5ul of culture medium to make the volume of the working solution to 1ml.
Similarly,
For 5ng/ml: Take 5ul of diluted stock (1ug/ml) and add to 995ul of culture medium to make the volume of the working solution to 1ml.
For 10ng/ml: Take 10ul of diluted stock (1ug/ml) and add to 990ul of culture medium to make the volume of the working solution to 1ml.
For 20ng/ml: Take 20ul of diluted stock (1ug/ml) and add to 980ul of culture medium to make the volume of the working solution to 1ml.
For 50ng/ml: Take 50ul of diluted stock (1ug/ml) and add to 950ul of culture medium to make the volume of the working solution to 1ml.
Use these working solutions for the experiment on the same day.
Good Luck.
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Louise Dolan you're welcome.
Louise Dolan you're welcome.
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