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DMPfold error while running protein structure prediction
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+ Bioinformatics
+ Biochemistry
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Melissa Zimmerman
DMPfold error while running protein structure prediction
Thanks, I did this but got this erro:
blastpgp -a 12 -b 0 -j 3 -h 0.001 -d c600m2-tail-before-lysin-sequence.a3m -i c600m2-tail-before-lysin-sequence.temp.fasta
[blastpgp] WARNING: Unable to open BLOSUM62
[blastpgp] WARNING: BlastScoreBlkMatFill returned non-zero status
[blastpgp] WARNING: SetUpBlastSearch failed.
BLASTP 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= C600M2_00100 tail protein [Salmonella phage BPS17S6]
(377 letters)
Database: c600m2-tail-before-lysin-sequence.a3m
46,978 sequences; 11,323,713 total letters
SearchingSegmentation fault (core dumped)
Could you please let me know how this could be solved? Thanks
blastpgp -a 12 -b 0 -j 3 -h 0.001 -d c600m2-tail-before-lysin-sequence.a3m -i c600m2-tail-before-lysin-sequence.temp.fasta[blastpgp] WARNING: Unable to open BLOSUM62[blastpgp] WARNING: BlastScoreBlkMatFill returned non-zero status[blastpgp] WARNING: SetUpBlastSearch failed.BLASTP 2.2.26 [Sep-21-2011]Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database searchprograms", Nucleic Acids Res. 25:3389-3402.Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searchesusing compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.Reference for composition-based statistics starting in round 2:Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.Query= C600M2_00100 tail protein [Salmonella phage BPS17S6] (377 letters)Database: c600m2-tail-before-lysin-sequence.a3m 46,978 sequences; 11,323,713 total lettersSearchingSegmentation fault (core dumped)
Could you please let me know how this could be solved? Thanks
Ok, I got it resolved by changing the correct blastpgp (legacy blast) executable. Thanks!More
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The comment by Devon Ryan is correct - looks like your blastpgp command is not working properly. For a protein of that length it's more likely to be a problem with the BLAST executable running on your system than a memory issue.
The comment by Devon Ryan is correct - looks like your blastpgp command is not working properly. For a protein of that length it's more likely to be a problem with the BLAST executable running on your system than a memory issue.
Thanks, I did this but got this erro:
Could you please let me know how this could be solved? Thanks
Thanks, I did this but got this erro:
Could you please let me know how this could be solved? Thanks
More
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The comment by Devon Ryan is correct - looks like your
blastpgpcommand is not working properly. For a protein of that length it's more likely to be a problem with the BLAST executable running on your system than a memory issue.Try running the
blastpgpcommand (https://github.com/psipred/DMPfold/blob/master/bin/runpsipredandsolvwithdb#L26) on its own and see what happens:The comment by Devon Ryan is correct - looks like your
blastpgpcommand is not working properly. For a protein of that length it's more likely to be a problem with the BLAST executable running on your system than a memory issue.Try running the
blastpgpcommand (https://github.com/psipred/DMPfold/blob/master/bin/runpsipredandsolvwithdb#L26) on its own and see what happens:More
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