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How can I dissolve hydrophobic compounds in DMEM media?
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+ Solubility
+ Cell culture techniques
+ Plant extracts
+ Dmso
+ Solution chemistry
+ Cytotoxicity assays
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Posted by
Mark Daly
How can I dissolve hydrophobic compounds in DMEM media?
I am experimenting with the plant extract containing high amount of decursin in in vitro cytotoxicity study. This compound is soluble in DMSO / 100% ethanol but completely insoluble in water. The prescribed dose for my study is (approximately 0 -10 mg), so when I apply DMSO solution of that compound to my cell culture media (obviously water based) at the desired dose, it forms a precipitate and makes the culture medium turbid. It will be very helpful if anybody can suggest a way out of this problem so that I can use this compound for my system.
