@jochen did you mean that we divide the conc (ng/ul) per 660 ? Or should we consider also the no. of base pairs of the plasmid? I am sorry but I am very confused.
If my linearized plasmid (7350bp) concentration is 100ng/ul, so the equation is like the following;
@jochen did you mean that we divide the conc (ng/ul) per 660 ? Or should we consider also the no. of base pairs of the plasmid? I am sorry but I am very confused.
If my linearized plasmid (7350bp) concentration is 100ng/ul, so the equation is like the following;
A simple formula is as follow: [(concentration in ng/ul0]/(660g/mol x average size of the of the dsDNA-determined by Bioanalyzer or Tapestation )] x 1000000 = Conc. in nM
A simple formula is as follow: [(concentration in ng/ul0]/(660g/mol x average size of the of the dsDNA-determined by Bioanalyzer or Tapestation )] x 1000000 = Conc. in nM
You don't just divide by 660. You divide by 660 g/mol bp. Mind the unit. If you take care about the units, it will be clear what's right and what's wrong. This should help you to resolve your confusion.
You don't just divide by 660. You divide by 660 g/mol bp. Mind the unit. If you take care about the units, it will be clear what's right and what's wrong. This should help you to resolve your confusion.
Thanks
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@jochen did you mean that we divide the conc (ng/ul) per 660 ? Or should we consider also the no. of base pairs of the plasmid?
I am sorry but I am very confused.
If my linearized plasmid (7350bp) concentration is 100ng/ul, so the equation is like the following;
(100÷ (660* 7350) )* 10^6 = conc.(nM) ?
Is that right?
@jochen did you mean that we divide the conc (ng/ul) per 660 ? Or should we consider also the no. of base pairs of the plasmid?
I am sorry but I am very confused.
If my linearized plasmid (7350bp) concentration is 100ng/ul, so the equation is like the following;
(100÷ (660* 7350) )* 10^6 = conc.(nM) ?
Is that right?
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You can assume an average molar mass of 660 g per bp.
You can assume an average molar mass of 660 g per bp.
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A simple formula is as follow:
[(concentration in ng/ul0]/(660g/mol x average size of the of the dsDNA-determined by Bioanalyzer or Tapestation )] x 1000000 = Conc. in nM
I hope this helps
A simple formula is as follow:
[(concentration in ng/ul0]/(660g/mol x average size of the of the dsDNA-determined by Bioanalyzer or Tapestation )] x 1000000 = Conc. in nM
I hope this helps
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You don't just divide by 660. You divide by 660 g/mol bp. Mind the unit. If you take care about the units, it will be clear what's right and what's wrong. This should help you to resolve your confusion.
You don't just divide by 660. You divide by 660 g/mol bp. Mind the unit. If you take care about the units, it will be clear what's right and what's wrong. This should help you to resolve your confusion.
More
VOTE