First Step Determine concentration of drug released by using formula Y =mX +/- C Y is absorbance, m is slope, C is intercept, X is concentration (mcg/ml) Second Step Calculate amount of drug released Amount of drug released (mg)= [Concentration X Dilution factor X Volume of Dissolution medium] / 1000 Final Step % Drug release = Amt of drug released (mg) X 100 / Dose (mg)
First Step Determine concentration of drug released by using formula Y =mX +/- C Y is absorbance, m is slope, C is intercept, X is concentration (mcg/ml) Second Step Calculate amount of drug released Amount of drug released (mg)= [Concentration X Dilution factor X Volume of Dissolution medium] / 1000 Final Step % Drug release = Amt of drug released (mg) X 100 / Dose (mg)
Formula for determination of percentage of release of drug from in vitro dissolution testing
- Concentration of drug (µg/ml)= (slope × absorbance) ± intercept
- Amount of drug = Concentration × Dissolution bath volume × dilution factor
released mg/ ml 1000- Cumulative percentage = Volume of sample withdrawn (ml) × P (t – 1) + Pt
release (%) Bath volume (v),Where Pt = Percentage release at time t
Where P (t – 1) = Percentage release previous to‘t’
Formula for determination of percentage of release of drug from in vitro dissolution testing
- Concentration of drug (µg/ml)= (slope × absorbance) ± intercept
- Amount of drug = Concentration × Dissolution bath volume × dilution factor
released mg/ ml 1000- Cumulative percentage = Volume of sample withdrawn (ml) × P (t – 1) + Pt
release (%) Bath volume (v),Where Pt = Percentage release at time t
Where P (t – 1) = Percentage release previous to‘t’
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Anna-Maria Stouki Thanks
Anna-Maria Stouki Thanks
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Dear Mr. Ali,
What is m in the equation of amount of drug release?
Dear Mr. Ali,
What is m in the equation of amount of drug release?
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Hazem Choukaife they changed from μg to mg, that's why.
Hazem Choukaife they changed from μg to mg, that's why.
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Check attached file
Check attached file
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Dose means how much mg we took for drug release?
Dose means how much mg we took for drug release?
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Wassam Ali how can we know amount of loading drug ?
Wassam Ali how can we know amount of loading drug ?
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Kailas K Mali
- Amount of drug = Concentration × Dissolution bath volume × dilution factor released mg/ ml 1000
for this equation, If I use impressed dried nanoparticles in release medium. in this situation, should I add dilution factor in the equation ??second why you divided the equation to 1000 ??
thank you
Kailas K Mali
- Amount of drug = Concentration × Dissolution bath volume × dilution factor released mg/ ml 1000
for this equation, If I use impressed dried nanoparticles in release medium. in this situation, should I add dilution factor in the equation ??second why you divided the equation to 1000 ??
thank you
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Dose is strength of tablet (Total quantity of API).
Check attached file
Dose is strength of tablet (Total quantity of API).
Check attached file
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Thanks all. I have really learnt. I am hoping the dose in Mr. Kailas equation means the theoretical total quantity of active ingredient in the tablet.
Thanks all. I have really learnt. I am hoping the dose in Mr. Kailas equation means the theoretical total quantity of active ingredient in the tablet.
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First Step
Determine concentration of drug released by using formula
Y =mX +/- C
Y is absorbance, m is slope, C is intercept, X is concentration (mcg/ml)
Second Step
Calculate amount of drug released
Amount of drug released (mg)= [Concentration X Dilution factor X Volume of Dissolution medium] / 1000
Final Step
% Drug release = Amt of drug released (mg) X 100 / Dose (mg)
First Step
Determine concentration of drug released by using formula
Y =mX +/- C
Y is absorbance, m is slope, C is intercept, X is concentration (mcg/ml)
Second Step
Calculate amount of drug released
Amount of drug released (mg)= [Concentration X Dilution factor X Volume of Dissolution medium] / 1000
Final Step
% Drug release = Amt of drug released (mg) X 100 / Dose (mg)
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VOTE
The following link explained the determining method of concentration using UV-Vis spectroscopy in a step by step manner:
http://www.analyzetest.com/index.php/2022/03/19/how-to-calculate-unknown-concentration-using-uv-vis-spectroscopy/
The following link explained the determining method of concentration using UV-Vis spectroscopy in a step by step manner:
http://www.analyzetest.com/index.php/2022/03/19/how-to-calculate-unknown-concentration-using-uv-vis-spectroscopy/
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