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How to use RNAProtect Bacterial to send samples for RNA extraction?
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+ Dna
+ Bacteria
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+ Rna purification
+ Molecular biology
+ Rna extraction
+ Molecular analysis
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Kevin Rome
How to use RNAProtect Bacterial to send samples for RNA extraction?
In that case Jessica Little the handbook on that page suggests 10exp8 cells should produce between 25 and 70ug RNA depending on the growth medium used so it looks like you can count cells,spin them down,decant most of the supenatant and then make up to a small convenient volume and add rnaprotect. You might contact the company making the rna for you and check with their tech support how and in what volume they want their samples sent. They will have a lot of expertise in converting various sample types and volumes to RNA
In that case Jessica Little the handbook on that page suggests 10exp8 cells should produce between 25 and 70ug RNA depending on the growth medium used so it looks like you can count cells,spin them down,decant most of the supenatant and then make up to a small convenient volume and add rnaprotect. You might contact the company making the rna for you and check with their tech support how and in what volume they want their samples sent. They will have a lot of expertise in converting various sample types and volumes to RNA
Paul Rutland Hi Paul, thank you for your response, however, the link you have posted isn't working? I also forgot to mention that this is for bacterial samples! Apologies for the confusion
Paul Rutland Hi Paul, thank you for your response, however, the link you have posted isn't working? I also forgot to mention that this is for bacterial samples! Apologies for the confusion
ok Jessica Little try the above text version. Links do seem to work less often since I changed to windows11 or possibly just search rnaprotect reagent formulation within RG
ok Jessica Little try the above text version. Links do seem to work less often since I changed to windows11 or possibly just search rnaprotect reagent formulation within RG
You will find some useful information in this discussion RNAprotect reagent formulation? (researchgate.net) which clarifies that due to the high density of RNAlater and rna protect the cells float and these reagents are not ideal for cell cultures. Tech help at qiagen are always very helpful
You will find some useful information in this discussion RNAprotect reagent formulation? (researchgate.net) which clarifies that due to the high density of RNAlater and rna protect the cells float and these reagents are not ideal for cell cultures. Tech help at qiagen are always very helpful
In that case Jessica Little the handbook on that page suggests 10exp8 cells should produce between 25 and 70ug RNA depending on the growth medium used so it looks like you can count cells,spin them down,decant most of the supenatant and then make up to a small convenient volume and add rnaprotect. You might contact the company making the rna for you and check with their tech support how and in what volume they want their samples sent. They will have a lot of expertise in converting various sample types and volumes to RNA
In that case Jessica Little the handbook on that page suggests 10exp8 cells should produce between 25 and 70ug RNA depending on the growth medium used so it looks like you can count cells,spin them down,decant most of the supenatant and then make up to a small convenient volume and add rnaprotect. You might contact the company making the rna for you and check with their tech support how and in what volume they want their samples sent. They will have a lot of expertise in converting various sample types and volumes to RNA
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Paul Rutland Hi Paul, thank you for your response, however, the link you have posted isn't working? I also forgot to mention that this is for bacterial samples! Apologies for the confusion
Paul Rutland Hi Paul, thank you for your response, however, the link you have posted isn't working? I also forgot to mention that this is for bacterial samples! Apologies for the confusion
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https://www.researchgate.net/post/RNAprotect_reagent_formulation#:~:text=RNAprotect%20Bacteria%20Reagent%3A%201%204%25%20%28w%2Fv%29%20Tetradecyl-trimethyl-ammonium%20bromide,mM%20tartaric%20acid%203%20NaOH%20to%20pH%204
ok Jessica Little try the above text version. Links do seem to work less often since I changed to windows11 or possibly just search rnaprotect reagent formulation within RG
https://www.researchgate.net/post/RNAprotect_reagent_formulation#:~:text=RNAprotect%20Bacteria%20Reagent%3A%201%204%25%20%28w%2Fv%29%20Tetradecyl-trimethyl-ammonium%20bromide,mM%20tartaric%20acid%203%20NaOH%20to%20pH%204
ok Jessica Little try the above text version. Links do seem to work less often since I changed to windows11 or possibly just search rnaprotect reagent formulation within RG
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You will find some useful information in this discussion
RNAprotect reagent formulation? (researchgate.net) which clarifies that due to the high density of RNAlater and rna protect the cells float and these reagents are not ideal for cell cultures. Tech help at qiagen are always very helpful
You will find some useful information in this discussion
RNAprotect reagent formulation? (researchgate.net) which clarifies that due to the high density of RNAlater and rna protect the cells float and these reagents are not ideal for cell cultures. Tech help at qiagen are always very helpful
More
VOTE
Paul Rutland Ah, I understand! Thank you very much for your answer. I was planning on the RNA Protect for Bacterial Samples:
https://www.qiagen.com/gb/products/discovery-and-translational-research/dna-rna-purification/rna-purification/total-rna/rnaprotect-reagents-and-tubes/?catno=76506
Paul Rutland Ah, I understand! Thank you very much for your answer. I was planning on the RNA Protect for Bacterial Samples:
https://www.qiagen.com/gb/products/discovery-and-translational-research/dna-rna-purification/rna-purification/total-rna/rnaprotect-reagents-and-tubes/?catno=76506
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