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Is it possible to substitute n-butanol with n-hexane in saponin...
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+ Saponins
+ Organic chemistry
+ Solvent extraction
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Misha Firer
Is it possible to substitute n-butanol with n-hexane in saponin...
Hello Lucas, No, you can´t substitute n-hexane instead of n-BuOH in saponin extraction. The n-hexane solven is not polar enough to solubilize the saponins, so you need use solvents with -OH groups (such as MeOH, EtOH, BuOH or H2O) to extract them.
Hello Lucas, No, you can´t substitute n-hexane instead of n-BuOH in saponin extraction. The n-hexane solven is not polar enough to solubilize the saponins, so you need use solvents with -OH groups (such as MeOH, EtOH, BuOH or H2O) to extract them.
Dear Lucas Herman, Replacing n-butanol with n-hexane has a negative effect on the extraction yield of saponins due to the fact that saponins have a hydrophilic and lipophylic moieties which interact efficiently with a solvent having a hydophilic and lipophylic moieties such as n-butanol. On the other hand n-hexane does not have any polar group to contribute to the solvation interactions with saponins. Hoping this will be helpful, Rafik
Dear Lucas Herman, Replacing n-butanol with n-hexane has a negative effect on the extraction yield of saponins due to the fact that saponins have a hydrophilic and lipophylic moieties which interact efficiently with a solvent having a hydophilic and lipophylic moieties such as n-butanol. On the other hand n-hexane does not have any polar group to contribute to the solvation interactions with saponins. Hoping this will be helpful, Rafik
Although this may not be a norm, most saponins extractions are performed on powdered plant material (various parts) using MeOH, ethanol (EtOH), water, or aqueous alcohol as extracting solvents. This is followed by a defatting (to remove lipophilic substances) step (generally with petroleum ether or n-hexane) which is carried out before the extraction step or on the extract itself. The extracts are then dissolved or suspended in water and shaken with n-butanol saturated with water. The n-butanol aliquots are then combined and the liquid removed to give crude saponin extract to work with. Some workers go yet a step further and opt for a precipitation stage using diethyl ether or acetone. Others go further still and include a dialysis stage to remove small water-soluble molecules, such as sugars. file:///C:/Users/M.%20PC/Downloads/Extraction%20and%20Isolation%20of%20Saponins.pdf
Although this may not be a norm, most saponins extractions are performed on powdered plant material (various parts) using MeOH, ethanol (EtOH), water, or aqueous alcohol as extracting solvents. This is followed by a defatting (to remove lipophilic substances) step (generally with petroleum ether or n-hexane) which is carried out before the extraction step or on the extract itself. The extracts are then dissolved or suspended in water and shaken with n-butanol saturated with water. The n-butanol aliquots are then combined and the liquid removed to give crude saponin extract to work with. Some workers go yet a step further and opt for a precipitation stage using diethyl ether or acetone. Others go further still and include a dialysis stage to remove small water-soluble molecules, such as sugars. file:///C:/Users/M.%20PC/Downloads/Extraction%20and%20Isolation%20of%20Saponins.pdf
no, you can not substitute, you can use methanol. methanol or ethanol will be the best. I suggest you go for sequential extraction from non-polar to polar.If u do sequential extraction you can remove Un wanted constituents.
no, you can not substitute, you can use methanol. methanol or ethanol will be the best. I suggest you go for sequential extraction from non-polar to polar.If u do sequential extraction you can remove Un wanted constituents.
I fully agree with previous researchers' comments. No. Because polarity of n-hexane differs from n- Butanol. You can select other solvent having similar polarity of Butanol.
I fully agree with previous researchers' comments. No. Because polarity of n-hexane differs from n- Butanol. You can select other solvent having similar polarity of Butanol.
Hello Lucas,
No, you can´t substitute n-hexane instead of n-BuOH in saponin extraction. The n-hexane solven is not polar enough to solubilize the saponins, so you need use solvents with -OH groups (such as MeOH, EtOH, BuOH or H2O) to extract them.
Hello Lucas,
No, you can´t substitute n-hexane instead of n-BuOH in saponin extraction. The n-hexane solven is not polar enough to solubilize the saponins, so you need use solvents with -OH groups (such as MeOH, EtOH, BuOH or H2O) to extract them.
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Saponins are molecules that present amphiphilic properties and to be extracted you need amphiphilic solvents such as n-butanol.
Saponins are molecules that present amphiphilic properties and to be extracted you need amphiphilic solvents such as n-butanol.
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Dear Lucas Herman,
Replacing n-butanol with n-hexane has a negative effect on the extraction yield of saponins due to the fact that saponins have a hydrophilic and lipophylic moieties which interact efficiently with a solvent having a hydophilic and lipophylic moieties such as n-butanol. On the other hand n-hexane does not have any polar group to contribute to the solvation interactions with saponins.
Hoping this will be helpful,
Rafik
Dear Lucas Herman,
Replacing n-butanol with n-hexane has a negative effect on the extraction yield of saponins due to the fact that saponins have a hydrophilic and lipophylic moieties which interact efficiently with a solvent having a hydophilic and lipophylic moieties such as n-butanol. On the other hand n-hexane does not have any polar group to contribute to the solvation interactions with saponins.
Hoping this will be helpful,
Rafik
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Although this may not be a norm, most saponins extractions are performed on powdered plant material (various parts) using MeOH, ethanol (EtOH), water, or aqueous alcohol as extracting solvents. This is followed by a defatting (to remove lipophilic substances) step (generally with petroleum ether or n-hexane) which is carried out before the extraction step or on the extract itself. The extracts are then dissolved or suspended in water and shaken with n-butanol saturated with water. The n-butanol aliquots are then combined and the liquid removed to give crude saponin extract to work with. Some workers go yet a step further and opt for a precipitation stage using diethyl ether or acetone. Others go further still and include a dialysis stage to remove small water-soluble molecules, such as sugars.
file:///C:/Users/M.%20PC/Downloads/Extraction%20and%20Isolation%20of%20Saponins.pdf
Although this may not be a norm, most saponins extractions are performed on powdered plant material (various parts) using MeOH, ethanol (EtOH), water, or aqueous alcohol as extracting solvents. This is followed by a defatting (to remove lipophilic substances) step (generally with petroleum ether or n-hexane) which is carried out before the extraction step or on the extract itself. The extracts are then dissolved or suspended in water and shaken with n-butanol saturated with water. The n-butanol aliquots are then combined and the liquid removed to give crude saponin extract to work with. Some workers go yet a step further and opt for a precipitation stage using diethyl ether or acetone. Others go further still and include a dialysis stage to remove small water-soluble molecules, such as sugars.
file:///C:/Users/M.%20PC/Downloads/Extraction%20and%20Isolation%20of%20Saponins.pdf
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no, you can not substitute, you can use methanol. methanol or ethanol will be the best. I suggest you go for sequential extraction from non-polar to polar.If u do sequential extraction you can remove Un wanted constituents.
no, you can not substitute, you can use methanol. methanol or ethanol will be the best. I suggest you go for sequential extraction from non-polar to polar.If u do sequential extraction you can remove Un wanted constituents.
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There are tremendous differences in the properties between the two solvents ,one is an alcohol, you just have to try.
There are tremendous differences in the properties between the two solvents ,one is an alcohol, you just have to try.
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I fully agree with previous researchers' comments.
No. Because polarity of n-hexane differs from n- Butanol. You can select other solvent having similar polarity of Butanol.
I fully agree with previous researchers' comments.
No. Because polarity of n-hexane differs from n- Butanol. You can select other solvent having similar polarity of Butanol.
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