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Is Sodium orthovanadate compatible with RIPA lysis buffer with EDTA?
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+ Edta
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Posted by
Mineta Harlow
Is Sodium orthovanadate compatible with RIPA lysis buffer with EDTA?
I am trying to detect protein phosphorylation by IP-IB. To prepare tissue lysates I am using RIPA lysis buffer which contains EDTA . I add Sodium Orthovanadate as a phosphatase inhibitor. I see in the data sheet that "The inhibition by Vanadate is completely reversible upon the addition of EDTA".Does it mean that sodium orthovanadate is inactive when I add it to my RIPA buffer?Thanks in advance.
