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Problems with colony PCR of Pichia pastoris - any thoughts?
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Posted by
Larry Hale
Problems with colony PCR of Pichia pastoris - any thoughts?
I need a protocol of colony PCR for confirmation of inserts after the Pichia pastoris electroporation. I performed a protocol, however, it didn't amplified any bands. So, I would like to compare to another one. Does somebody has any protocol (from collect colony until the pcr mix) for colony PCR of P. pastoris transformants?
