Home >
Community >
qPCR: do both primers have to contain a GC-clamp in order to be effective?
Upvote
27
Downvote
+ Pcr
+ Primer
Posted by
Michel Paul
qPCR: do both primers have to contain a GC-clamp in order to be effective?
You can have GC-clamps (3' terminal G/C) in both primers; this may overall improve the PCR efficiency. However you should take care not to put too many G/Cs. This may lead to stabilization of primer pairs, leading to formation of primer-dimers. Also see this post: When designing primers how important is the GC clamp?
GC-clamps are not always essential and you should look at other parameters too.
You can have GC-clamps (3' terminal G/C) in both primers; this may overall improve the PCR efficiency. However you should take care not to put too many G/Cs. This may lead to stabilization of primer pairs, leading to formation of primer-dimers. Also see this post: When designing primers how important is the GC clamp?
GC-clamps are not always essential and you should look at other parameters too.
So, in summary: A clamp in one primer is good for efficiency and clamps in both are better (with the right amount of G/C ofcourse). So I don't need a GC-clamp in both of them. Is this right?More
You can have GC-clamps (3' terminal
G/C) in both primers; this may overall improve the PCR efficiency. However you should take care not to put too manyG/Cs. This may lead to stabilization of primer pairs, leading to formation of primer-dimers. Also see this post: When designing primers how important is the GC clamp?GC-clamps are not always essential and you should look at other parameters too.
You can have GC-clamps (3' terminal
G/C) in both primers; this may overall improve the PCR efficiency. However you should take care not to put too manyG/Cs. This may lead to stabilization of primer pairs, leading to formation of primer-dimers. Also see this post: When designing primers how important is the GC clamp?GC-clamps are not always essential and you should look at other parameters too.
More
VOTE
VOTE
VOTE