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What happens if both forward and reverse primers have same Tm?
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Pamela Anne Trinidad
What happens if both forward and reverse primers have same Tm?
It is actually good if both the primers have the same Tm (Melting Temperature) because you would basically want both primers to anneal at your Ta (Annealing Temperature) which is generally set as Tm-5. If the Tm(s) don't match, then your Ta should be set according to the lower Tm.
It is actually good if both the primers have the same Tm (Melting Temperature) because you would basically want both primers to anneal at your Ta (Annealing Temperature) which is generally set as Tm-5. If the Tm(s) don't match, then your Ta should be set according to the lower Tm.
It is actually good if both the primers have the same
Tm(Melting Temperature) because you would basically want both primers to anneal at yourTa(Annealing Temperature) which is generally set asTm-5. If theTm(s)don't match, then yourTashould be set according to the lowerTm.It is actually good if both the primers have the same
Tm(Melting Temperature) because you would basically want both primers to anneal at yourTa(Annealing Temperature) which is generally set asTm-5. If theTm(s)don't match, then yourTashould be set according to the lowerTm.More
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