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What is the best way to adjust the pH of an ammonium acetate...
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+ High-performance liquid chromatography
+ Buffer preparation
+ Chromatography
Posted by
Matthew Arslan
What is the best way to adjust the pH of an ammonium acetate...
1) When preparing an ammonium acetate 5mM buffer solution with pH=3.3, which is better to use to adjust the pH? An inorganic acid (HCl) or the corresponding organic acid (CH3COOH)?2) Is it necessary to prepare the buffer solution using ultra pure water or distilled water is tolerated? because it will be used for an HPLC C18 column injection.Aliphatic carboxylic diacids, such as glutaric and succinc acids, will be injected.
