Do not use bleach, or NaOH, HCl, keep pH between 2-13. Yet you can use pyridine or NaHCO3 dilute solution in some case. But in ordinary MeOH with small amount acetic acid washing is enough to wash for LH20 column chromatography. Any material which is not dissolved in MeOH cleanly should not be applied , or filtrate before charge. LH20 can be used in EtOH, EtOH/water, acetone etc, however. Yet it is good to separate plant aromatic compounds soluble to MeOH.
Do not use bleach, or NaOH, HCl, keep pH between 2-13. Yet you can use pyridine or NaHCO3 dilute solution in some case. But in ordinary MeOH with small amount acetic acid washing is enough to wash for LH20 column chromatography. Any material which is not dissolved in MeOH cleanly should not be applied , or filtrate before charge. LH20 can be used in EtOH, EtOH/water, acetone etc, however. Yet it is good to separate plant aromatic compounds soluble to MeOH.
Do not use bleach, or NaOH, HCl, keep pH between 2-13. Yet you can use pyridine or NaHCO3 dilute solution in some case. But in ordinary MeOH with small amount acetic acid washing is enough to wash for LH20 column chromatography. Any material which is not dissolved in MeOH cleanly should not be applied , or filtrate before charge. LH20 can be used in EtOH, EtOH/water, acetone etc, however. Yet it is good to separate plant aromatic compounds soluble to MeOH.
Do not use bleach, or NaOH, HCl, keep pH between 2-13. Yet you can use pyridine or NaHCO3 dilute solution in some case. But in ordinary MeOH with small amount acetic acid washing is enough to wash for LH20 column chromatography. Any material which is not dissolved in MeOH cleanly should not be applied , or filtrate before charge. LH20 can be used in EtOH, EtOH/water, acetone etc, however. Yet it is good to separate plant aromatic compounds soluble to MeOH.
As a general guide, keep the pH between 2-13 and avoid strong oxidizers.
As a general guide, keep the pH between 2-13 and avoid strong oxidizers.
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It is better than using bleach but pH must be maintained in desired limit.
It is better than using bleach but pH must be maintained in desired limit.
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Ask the manufacturer. Always ask the manufacturer first. If what they suggest doesn't work, then go and ask the internet.
Ask the manufacturer. Always ask the manufacturer first. If what they suggest doesn't work, then go and ask the internet.
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Sure , while sodium hypochlorite has active chlorine which will react with sephadex
Sure , while sodium hypochlorite has active chlorine which will react with sephadex
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Do not use bleach, or NaOH, HCl, keep pH between 2-13. Yet you can use pyridine or NaHCO3 dilute solution in some case. But in ordinary MeOH with small amount acetic acid washing is enough to wash for LH20 column chromatography. Any material which is not dissolved in MeOH cleanly should not be applied , or filtrate before charge. LH20 can be used in EtOH, EtOH/water, acetone etc, however. Yet it is good to separate plant aromatic compounds soluble to MeOH.
Do not use bleach, or NaOH, HCl, keep pH between 2-13. Yet you can use pyridine or NaHCO3 dilute solution in some case. But in ordinary MeOH with small amount acetic acid washing is enough to wash for LH20 column chromatography. Any material which is not dissolved in MeOH cleanly should not be applied , or filtrate before charge. LH20 can be used in EtOH, EtOH/water, acetone etc, however. Yet it is good to separate plant aromatic compounds soluble to MeOH.
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Do not use bleach, or NaOH, HCl, keep pH between 2-13. Yet you can use pyridine or NaHCO3 dilute solution in some case. But in ordinary MeOH with small amount acetic acid washing is enough to wash for LH20 column chromatography. Any material which is not dissolved in MeOH cleanly should not be applied , or filtrate before charge. LH20 can be used in EtOH, EtOH/water, acetone etc, however. Yet it is good to separate plant aromatic compounds soluble to MeOH.
Do not use bleach, or NaOH, HCl, keep pH between 2-13. Yet you can use pyridine or NaHCO3 dilute solution in some case. But in ordinary MeOH with small amount acetic acid washing is enough to wash for LH20 column chromatography. Any material which is not dissolved in MeOH cleanly should not be applied , or filtrate before charge. LH20 can be used in EtOH, EtOH/water, acetone etc, however. Yet it is good to separate plant aromatic compounds soluble to MeOH.
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add a small zone of DMSO and elute is using methanol or MeOH/Water
add a small zone of DMSO and elute is using methanol or MeOH/Water
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