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What is the effective way of crosslinking gelatin solution with...
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+ Polymer science
+ Polymerization
+ Freeze drying
+ Gelatin
+ Tissue engineering
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Mekonnen Assegu
What is the effective way of crosslinking gelatin solution with...
EDC and NHS activation is ofte performed at pH5-7 in MES buffer, and the reaction of an amine with the activated carboxylic acid works well in PBS at pH=7-8, so I would say in a one step process where both the -COOH and -NH2 are present, just adding the EDC/HNS to a buffered solution close to physiological pH of the gelatin should work fine. Give it a go. If it is too slow, start off at slightly lower pH (say 6) and increase it after a few minutes of activation to 7.4 (before it crosslinks). Not sure what shape you want, but in stead of finding teflon-coatred moulds or coating moulds with Teflon, you could machine moulds directly out of Teflon stock.
EDC and NHS activation is ofte performed at pH5-7 in MES buffer, and the reaction of an amine with the activated carboxylic acid works well in PBS at pH=7-8, so I would say in a one step process where both the -COOH and -NH2 are present, just adding the EDC/HNS to a buffered solution close to physiological pH of the gelatin should work fine. Give it a go. If it is too slow, start off at slightly lower pH (say 6) and increase it after a few minutes of activation to 7.4 (before it crosslinks). Not sure what shape you want, but in stead of finding teflon-coatred moulds or coating moulds with Teflon, you could machine moulds directly out of Teflon stock.
I hope you had good results with your approach. Currently, I am trying to use a similar method for my work. I hope you can share your protocol or published work with me.
I hope you had good results with your approach. Currently, I am trying to use a similar method for my work. I hope you can share your protocol or published work with me.
EDC and NHS activation is ofte performed at pH5-7 in MES buffer, and the reaction of an amine with the activated carboxylic acid works well in PBS at pH=7-8, so I would say in a one step process where both the -COOH and -NH2 are present, just adding the EDC/HNS to a buffered solution close to physiological pH of the gelatin should work fine. Give it a go. If it is too slow, start off at slightly lower pH (say 6) and increase it after a few minutes of activation to 7.4 (before it crosslinks). Not sure what shape you want, but in stead of finding teflon-coatred moulds or coating moulds with Teflon, you could machine moulds directly out of Teflon stock.
EDC and NHS activation is ofte performed at pH5-7 in MES buffer, and the reaction of an amine with the activated carboxylic acid works well in PBS at pH=7-8, so I would say in a one step process where both the -COOH and -NH2 are present, just adding the EDC/HNS to a buffered solution close to physiological pH of the gelatin should work fine. Give it a go. If it is too slow, start off at slightly lower pH (say 6) and increase it after a few minutes of activation to 7.4 (before it crosslinks). Not sure what shape you want, but in stead of finding teflon-coatred moulds or coating moulds with Teflon, you could machine moulds directly out of Teflon stock.
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Hello Emmanuel,
I hope you had good results with your approach. Currently, I am trying to use a similar method for my work. I hope you can share your protocol or published work with me.
Thanks in advance
Hello Emmanuel,
I hope you had good results with your approach. Currently, I am trying to use a similar method for my work. I hope you can share your protocol or published work with me.
Thanks in advance
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