From my point of view: 1- For isolation: extract with MeOH. Defatting with diethyl ether or CH2Cl2. Fractionation of the defatted extract on Diaion HP-20 using first water then 40% MeOH, 80% MeOH and finally MeOH. Saponin rich fraction is fractionated by silica gel and RP-18 and finally by HPLC 2- Characterization: by different NMR & MS analyses Keep in mind if you have steroidal saponins of furostanol type, the conversion of 22-OH group to its OMe derivative due to use of MeOH in the extraction and chromatographic processes
From my point of view: 1- For isolation: extract with MeOH. Defatting with diethyl ether or CH2Cl2. Fractionation of the defatted extract on Diaion HP-20 using first water then 40% MeOH, 80% MeOH and finally MeOH. Saponin rich fraction is fractionated by silica gel and RP-18 and finally by HPLC 2- Characterization: by different NMR & MS analyses Keep in mind if you have steroidal saponins of furostanol type, the conversion of 22-OH group to its OMe derivative due to use of MeOH in the extraction and chromatographic processes
http://link.springer.com/protocol/10.1007%2F978-1-61779-624-1_16
http://link.springer.com/protocol/10.1007%2F978-1-61779-624-1_16
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The attached article could be of help.
Best regards
Robert
The attached article could be of help.
Best regards
Robert
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i agree with Mohammed Kamel
i agree with Mohammed Kamel
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From my point of view:
1- For isolation: extract with MeOH. Defatting with diethyl ether or CH2Cl2. Fractionation of the defatted extract on Diaion HP-20 using first water then 40% MeOH, 80% MeOH and finally MeOH. Saponin rich fraction is fractionated by silica gel and RP-18 and finally by HPLC
2- Characterization: by different NMR & MS analyses
Keep in mind if you have steroidal saponins of furostanol type, the conversion of 22-OH group to its OMe derivative due to use of MeOH in the extraction and chromatographic processes
From my point of view:
1- For isolation: extract with MeOH. Defatting with diethyl ether or CH2Cl2. Fractionation of the defatted extract on Diaion HP-20 using first water then 40% MeOH, 80% MeOH and finally MeOH. Saponin rich fraction is fractionated by silica gel and RP-18 and finally by HPLC
2- Characterization: by different NMR & MS analyses
Keep in mind if you have steroidal saponins of furostanol type, the conversion of 22-OH group to its OMe derivative due to use of MeOH in the extraction and chromatographic processes
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Check these RG threads on the subject and publications on saponins
https://www.researchgate.net/post/how_could_be_assayed_the_total_saponins_in_a_plant_extract
https://www.researchgate.net/post/Does_anyone_know_about_saponins_which_have_the_general_range_of_thermal_stability?tpr_view=8eab4d88-65bb-493d-8628-e504e904659e_1
https://www.researchgate.net/post/How_do_I_obtain_crude_saponin_extract_from_my_plant_extract2?tpr_view=d45502f4-5bd9-4750-8f68-656027460ee8_2
https://www.researchgate.net/post/What_is_a_suitable_method_of_hydrolysis_of_saponin_glycosides_to_get_the_sapogenin_from_natural_products
https://www.researchgate.net/post/What_is_the_most_efficient_method_for_extraction_of_phytochemicals_from_plants
Check these RG threads on the subject and publications on saponins
https://www.researchgate.net/post/how_could_be_assayed_the_total_saponins_in_a_plant_extract
https://www.researchgate.net/post/Does_anyone_know_about_saponins_which_have_the_general_range_of_thermal_stability?tpr_view=8eab4d88-65bb-493d-8628-e504e904659e_1
https://www.researchgate.net/post/How_do_I_obtain_crude_saponin_extract_from_my_plant_extract2?tpr_view=d45502f4-5bd9-4750-8f68-656027460ee8_2
https://www.researchgate.net/post/What_is_a_suitable_method_of_hydrolysis_of_saponin_glycosides_to_get_the_sapogenin_from_natural_products
https://www.researchgate.net/post/What_is_the_most_efficient_method_for_extraction_of_phytochemicals_from_plants
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