Spectrophotometric and liquid chromatographic methods are usually employed to determine saponins. The vanillin-sulfuric acid assay is the most selected spectrophotometric method for saponin quantification since it is simple, fast and inexpensive to operate. The basic principle of this method is the reaction of sulphuric acid-oxidized triterpene saponins with vanillin, which gives a distinctive red-purple color measured at wavelengths ranging from 475 to 560 nm using a spectrophotometer.
Please refer to the review article attached below in which the quantification methods of saponins namely, spectrophotometric and chromatographic, are summarized and discussed.
Spectrophotometric and liquid chromatographic methods are usually employed to determine saponins. The vanillin-sulfuric acid assay is the most selected spectrophotometric method for saponin quantification since it is simple, fast and inexpensive to operate. The basic principle of this method is the reaction of sulphuric acid-oxidized triterpene saponins with vanillin, which gives a distinctive red-purple color measured at wavelengths ranging from 475 to 560 nm using a spectrophotometer.
Please refer to the review article attached below in which the quantification methods of saponins namely, spectrophotometric and chromatographic, are summarized and discussed.
I haven't worked around this chemical material, but I can check with the suppliers of the materials for this kit
I haven't worked around this chemical material, but I can check with the suppliers of the materials for this kit
More
VOTE
Yes, there is: e.g. https://www.biorbyt.com/saponin-microplate-assay-kit-orb759224.html
Yes, there is: e.g. https://www.biorbyt.com/saponin-microplate-assay-kit-orb759224.html
More
VOTE
Hello Nadia Rachdaoui
Spectrophotometric and liquid chromatographic methods are usually employed to determine saponins. The vanillin-sulfuric acid assay is the most selected spectrophotometric method for saponin quantification since it is simple, fast and inexpensive to operate. The basic principle of this method is the reaction of sulphuric acid-oxidized triterpene saponins with vanillin, which gives a distinctive red-purple color measured at wavelengths ranging from 475 to 560 nm using a spectrophotometer.
Please refer to the review article attached below in which the quantification methods of saponins namely, spectrophotometric and chromatographic, are summarized and discussed.
https://www.sciencedirect.com/science/article/abs/pii/S096399691400074X?via%3Dihub
Best.
Hello Nadia Rachdaoui
Spectrophotometric and liquid chromatographic methods are usually employed to determine saponins. The vanillin-sulfuric acid assay is the most selected spectrophotometric method for saponin quantification since it is simple, fast and inexpensive to operate. The basic principle of this method is the reaction of sulphuric acid-oxidized triterpene saponins with vanillin, which gives a distinctive red-purple color measured at wavelengths ranging from 475 to 560 nm using a spectrophotometer.
Please refer to the review article attached below in which the quantification methods of saponins namely, spectrophotometric and chromatographic, are summarized and discussed.
https://www.sciencedirect.com/science/article/abs/pii/S096399691400074X?via%3Dihub
Best.
More
VOTE