Total saponin content was determined through spectrophotometry, as described by Medina-Meza, Aluwi, Saunders, and Ganjyal (2016). Briefly, 0.25 ml of saponin extract of each sample solution was added to 1 ml of reagent mixture (glacial acetic acid/sulfuric acid 1:1 v/v). The mixture was vortexed and reacted at 60°C in a water bath for 30 min and then cooled. The absorbance of the sample was measured at a wavelength of 527 nm using a spectrophotometer (Multiskan GO, Thermo Scientific). Oleanolic acid was used as a standard (0–1,000 μg/ml). Total saponin content was expressed as g 100 g−1 of oleanolic acid equivalents.
Total saponin content was determined through spectrophotometry, as described by Medina-Meza, Aluwi, Saunders, and Ganjyal (2016). Briefly, 0.25 ml of saponin extract of each sample solution was added to 1 ml of reagent mixture (glacial acetic acid/sulfuric acid 1:1 v/v). The mixture was vortexed and reacted at 60°C in a water bath for 30 min and then cooled. The absorbance of the sample was measured at a wavelength of 527 nm using a spectrophotometer (Multiskan GO, Thermo Scientific). Oleanolic acid was used as a standard (0–1,000 μg/ml). Total saponin content was expressed as g 100 g−1 of oleanolic acid equivalents.
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Hi, Gaëtan,
If you want to determine total saponin content, you should need saponin standards as analytical reference
Best regards
Hi, Gaëtan,
If you want to determine total saponin content, you should need saponin standards as analytical reference
Best regards
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Total saponin content was determined through spectrophotometry,
as described by Medina-Meza, Aluwi, Saunders, and Ganjyal
(2016). Briefly, 0.25 ml of saponin extract of each sample solution
was added to 1 ml of reagent mixture (glacial acetic acid/sulfuric acid
1:1 v/v). The mixture was vortexed and reacted at 60°C in a water
bath for 30 min and then cooled. The absorbance of the sample
was measured at a wavelength of 527 nm using a spectrophotometer
(Multiskan GO, Thermo Scientific). Oleanolic acid was used as a
standard (0–1,000 μg/ml). Total saponin content was expressed as g
100 g−1 of oleanolic acid equivalents.
Total saponin content was determined through spectrophotometry,
as described by Medina-Meza, Aluwi, Saunders, and Ganjyal
(2016). Briefly, 0.25 ml of saponin extract of each sample solution
was added to 1 ml of reagent mixture (glacial acetic acid/sulfuric acid
1:1 v/v). The mixture was vortexed and reacted at 60°C in a water
bath for 30 min and then cooled. The absorbance of the sample
was measured at a wavelength of 527 nm using a spectrophotometer
(Multiskan GO, Thermo Scientific). Oleanolic acid was used as a
standard (0–1,000 μg/ml). Total saponin content was expressed as g
100 g−1 of oleanolic acid equivalents.
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Dear Gaetan Richard, find the attachment
Dear Gaetan Richard, find the attachment
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Dear Gaetan Richard, find the attachment
Dear Gaetan Richard, find the attachment
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