Thanks Romy for your valuable advice. I got your point and it should be fine since I usually stimulate the cells then stain for surface markers. I have experienced problems with tandem conjugates. Are they stable in Na-azide-containing mix and for how long?
Thanks Romy for your valuable advice. I got your point and it should be fine since I usually stimulate the cells then stain for surface markers. I have experienced problems with tandem conjugates. Are they stable in Na-azide-containing mix and for how long?
I would not only use PBS+BSA but add some sodium azide. (1x PBS+0,1% BSA+0,02% NaN3) Then it is possible to use this cocktail for at least one month, in this buffer and as well in saponin or another perm-buffer. But take care if you want to do stimulation and stain surface markers beforestimulation. In this case your buffer should not contain NaN3 because then, the cells don't want to produce cytokines any more. The other way around, first stimulation and then surface staining is possible with antibody-cocktails in buffer with NaN3.
I would not only use PBS+BSA but add some sodium azide. (1x PBS+0,1% BSA+0,02% NaN3) Then it is possible to use this cocktail for at least one month, in this buffer and as well in saponin or another perm-buffer. But take care if you want to do stimulation and stain surface markers beforestimulation. In this case your buffer should not contain NaN3 because then, the cells don't want to produce cytokines any more. The other way around, first stimulation and then surface staining is possible with antibody-cocktails in buffer with NaN3.
Thanks Sudhanshu for your answer
Thanks Sudhanshu for your answer
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Thanks Romy for your valuable advice. I got your point and it should be fine since I usually stimulate the cells then stain for surface markers. I have experienced problems with tandem conjugates. Are they stable in Na-azide-containing mix and for how long?
Thanks Romy for your valuable advice. I got your point and it should be fine since I usually stimulate the cells then stain for surface markers. I have experienced problems with tandem conjugates. Are they stable in Na-azide-containing mix and for how long?
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If you make a cocktail of antibodies in PBS-BSA, it's ok to store it in the refrigerator for 1-2 days but not longer. Hope it helps :)
If you make a cocktail of antibodies in PBS-BSA, it's ok to store it in the refrigerator for 1-2 days but not longer. Hope it helps :)
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I would not only use PBS+BSA but add some sodium azide. (1x PBS+0,1% BSA+0,02% NaN3)
Then it is possible to use this cocktail for at least one month, in this buffer and as well in saponin or another perm-buffer.
But take care if you want to do stimulation and stain surface markers before stimulation. In this case your buffer should not contain NaN3 because then, the cells don't want to produce cytokines any more. The other way around, first stimulation and then surface staining is possible with antibody-cocktails in buffer with NaN3.
I would not only use PBS+BSA but add some sodium azide. (1x PBS+0,1% BSA+0,02% NaN3)
Then it is possible to use this cocktail for at least one month, in this buffer and as well in saponin or another perm-buffer.
But take care if you want to do stimulation and stain surface markers before stimulation. In this case your buffer should not contain NaN3 because then, the cells don't want to produce cytokines any more. The other way around, first stimulation and then surface staining is possible with antibody-cocktails in buffer with NaN3.
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VOTE