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Why am I getting less than 100% recovery after solid phase extraction?
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Posted by
Mike Isbad
Why am I getting less than 100% recovery after solid phase extraction?
I dissolved diazepam in acetonitrile and got a concentration of 1 ppm. after SPE, then ran the sample through HPLC. Total % recovery (conditioning, loading, washing, eluting) didn't reach 100%. I have eluted up to 15 times and on the 7th eluting the AUC was 0.000. Where's the rest of the diazepam?Cond: H2O:ACN (9:1)Loding: Diazepam in acetonitrileWashing :H2OEluting : Methanol: Acetic Acid (8:2)
