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Did anybody experience clogged spin columns with the Norgen total...
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Neal Pepper
Did anybody experience clogged spin columns with the Norgen total...
I know you asked this questions a long time ago but I share my own experience to help others facing the same problem. Yes, I have experienced clogged columns 3-4 times, three times after adding lysis buffer and once after wash. I have read before that some of Norgen columns have this problem due to the type of silica they have used in columns.However I think the lysis buffer must be preheated before use. I guess this step should not be skipped even if no precipitate is observed in the buffer.
I know you asked this questions a long time ago but I share my own experience to help others facing the same problem. Yes, I have experienced clogged columns 3-4 times, three times after adding lysis buffer and once after wash. I have read before that some of Norgen columns have this problem due to the type of silica they have used in columns.However I think the lysis buffer must be preheated before use. I guess this step should not be skipped even if no precipitate is observed in the buffer.
I do not know how you are pretreating the preparation of the RNA (which you must do before you load the wells of the spin column), but in general I will advise that you do not use all the 96 wells (if it is getting clogged). May be you can use only 80 wells and that too in random.
I do not know how you are pretreating the preparation of the RNA (which you must do before you load the wells of the spin column), but in general I will advise that you do not use all the 96 wells (if it is getting clogged). May be you can use only 80 wells and that too in random.
I know you asked this questions a long time ago but I share my own experience to help others facing the same problem. Yes, I have experienced clogged columns 3-4 times, three times after adding lysis buffer and once after wash. I have read before that some of Norgen columns have this problem due to the type of silica they have used in columns.However I think the lysis buffer must be preheated before use. I guess this step should not be skipped even if no precipitate is observed in the buffer.
I know you asked this questions a long time ago but I share my own experience to help others facing the same problem. Yes, I have experienced clogged columns 3-4 times, three times after adding lysis buffer and once after wash. I have read before that some of Norgen columns have this problem due to the type of silica they have used in columns.However I think the lysis buffer must be preheated before use. I guess this step should not be skipped even if no precipitate is observed in the buffer.
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I do not know how you are pretreating the preparation of the RNA (which you must do before you load the wells of the spin column), but in general I will advise that you do not use all the 96 wells (if it is getting clogged). May be you can use only 80 wells and that too in random.
I do not know how you are pretreating the preparation of the RNA (which you must do before you load the wells of the spin column), but in general I will advise that you do not use all the 96 wells (if it is getting clogged). May be you can use only 80 wells and that too in random.
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