Disodium ethylenediaminetetraacetate

Disodium ethylenediaminetetraacetate is water soluble form of EDTA, because its chelating ability of divalent metal ions, and is widely used in biochemical research. This chelating property is crucial in a variety of scientific studies, especially in maintaining biochemical and molecular integrity by inhibiting enzymatic processes that rely on metal ions. In the study, disodium ethylenediaminetetraacetate used for isolation of metal ions, such as calcium and magnesium, these metal ions are many enzymes, including DNase and RNase cofactors necessary. By combining these ions, disodium ethylenediaminetetraacetate can prevent the enzyme activity, nucleic acid or enzyme activity will be in the process of extracting degradation, thus ensuring the stability of DNA and RNA samples and purity. In addition, its application extends to analytical chemistry, where it is used in complexometric titration to determine the hardness of water or to quantify the concentration of metal ions in a solution. In cell biology, disodium ethylenediaminetetraacetate is the key component of the cell culture medium, for by chelating calcium ion, damage cells and between cells and the interaction between cells and matrix to promote adhesion cell separation. This wide range of uses in binding metal ions not only underscores its importance in laboratory protocols, but also contributes to its wide application in the study of metal proteins and other metal-dependent biological phenomena.