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Protein Research


It can be supplemented with iron or used to bind free iron in the culture medium. Used to transport iron into mammalian cells.


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N-α-Tosyl-L-phenylalanylchloromethane is a proteinase inhibitors with apoptotic function. Studies have shown that it induces caspase-dependent apoptosis in Epstein-Barr virus (EBV)-transformed human B cell lines with release of pro-apoptotic proteins from mitochondria. It also results in down-regulation of the anti-apoptotic proteins and caspase-dependent cleavage of two anti-apoptotic proteins. It promotes dephosphorylation of p53 on serine residues.

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Ferritin is an iron-containing protein with electron-dense characteristics, which can accurately locate cell antigens and can be seen under an electron microscope. Using the principle that antigen and antibody specifically bind to produce a small amount of precipitation, the ultrastructural identification and positioning of antigen and antibody in tissue cells are performed. Therefore, ferritin has a broad future in medical and biological research.

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Carboxypeptidase A(CPA) is an enzyme of the digestive system that is known to cleave amino acids favouring the C-terminal end as well as certain esters. This enzymatic activity depends on the metal at the catalytic site. Zn
and some Co
-containing CPAs exhibit peptidase function, whilst esterase function has been seen by CPAs containing a variety of divalent d-block metals. CPA has a size similar to CA, consisting of about 300 amino acids and a molecular mass of 34 kDa.


Tosyllysine chloromethylketone (TLCK) is an active site-directed agent that inhibits serine proteinases with trypsin-like activity. TLCK may also act non-selectively with thiol groups and thereby inhibit cysteine proteinases and other enzymes. To prevent proteolytic degradation, TLCK may be used in protein purification protocols. TLCK selectively inactiviates clostripain obtained from C. histolyticum.

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Protein engineering mainly studies protein separation and purification, protein structure and function analysis, design and prediction, and transforms and creates proteins through genetic engineering methods. The study of the relationship between protein structure and function is the core content of protein engineering. With the rapid development of molecular biology, structural biology and bioinformatics, protein engineering has developed rapidly in recent years and has become an important means of studying protein structure and function. It is also widely used in protein research and drug design.
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