The advantages of biological buffer Tris and precautions for use

Biological buffers are of great significance in biochemical research, and are widely used in experiments such as western blotting, biochips and biological hybridization, and in vitro diagnostic industries. The biological buffer system contains 20-30 varieties, and Desun’s main biological buffer covers the most important products. Such as Tris, HEPES, MOPS, CAPS, Tris-HCl, etc. This article will introduce the advantages and precautions of Tris buffer, which is the advantage product of Desheng. 

The full name of Tris is tris(hydroxymethyl)aminomethane, the CAS number is 77-86-1, the pKa at 25°C is 8.06, and the buffer range is 7.0-9.0. Common Tris buffers include Tris-HCl buffer, Tris-phosphate buffer and a variety of derivative buffers, such as TBS, TE, etc. Tris buffer is a very important biological buffer, its advantages are as follows:

1. Tris base is relatively alkaline, so this buffer system can be used to prepare a wide range of pH buffers ranging from acidic to alkaline;

2. It has little interference to the biochemical process, and does not precipitate with calcium, magnesium ions and heavy metal ions;

3. High solubility in water and inert to many enzyme reactions;

4. High buffering capacity, generally used to stabilize the pH of the reaction system, with strong buffering capacity between pH 7.5-9.0;

5. Tris buffers are widely used and play an important role in biochemistry, molecular biology, in vitro diagnostics, cosmetics, coatings and other fields.

Matters needing attention when using Tris buffer:

1. The pH value of Tris buffer is greatly affected by temperature and concentration, so the use environment should be considered during the preparation process;

2. Tris reacts with various molecules to a certain extent, including RNase inhibitors, aldehydes, enzymes, DNA, and common metals such as Cr3+, Fe3+, Ni2+, Co2+ and Cu2+, etc. It can work with heavy metals, but in some systems Also inhibits

3. It is easy to absorb CO2 in the air, and the prepared buffer should be tightly sealed;

4. It has certain interference effects on some pH electrodes, and electrodes that are compatible with Tris solution are required;

5. Tris is not suitable for bicinchoninic acid (BCA) determination;

6. Inhalation, ingestion or skin absorption can cause injury. Wear gloves and goggles during operation.

Desheng’s main product biological buffer is a kind of alcohol-ammonia fine chemicals with special characteristics. It does not participate in and does not interfere with the biochemical process. It can be used as a buffer system for life science research and provides a stable pH environment for experiments. Desheng biological buffer Agents are widely used in in vitro diagnostics, biopharmaceuticals, biological cosmetics, paints and paints and other industries. Due to the high buffering efficiency and quality of the products, there are now dozens of stable customers. Desheng provides one-stop procurement services. If needed, please click on the official website to consult customer service.