Since the outbreak of the new coronavirus, the virus preservation solution has played a major role. First, nasal and throat swabs are used to sample samples, and the samples are placed in a sampling tube with virus preservation solution for sample protection, which improves the accuracy of nucleic acid detection. The virus preservation solution is a solution medium used for virus sampling, transportation, preservation, and detection. It is added to the virus sampling tube. What kind of virus preservation solution is added?
There are two types of virus preservation solutions, inactivated and non-inactivated. There are many types of virus preservation solutions on the market. The following Desheng editor briefly introduces several principles of virus preservation solution selection.
Non-inactivated preservation solution:
The non-inactivated type does not contain lysis solution. On the basis of Hank's, it is added with BSA (bovine serum albumin) amino acids, vitamins and other nutrients required by the virus, which can maintain the activity of the virus in a wide temperature range and maintain the maximum The originality of the sample can be used for virus nucleic acid extraction and detection, virus cultivation and isolation.
Features of non-inactivated preservation solution:
1. The virus can remain active and can be used for detection or virus isolation;
2. It has no inhibition on subsequent nucleic acid amplification and can be matched with direct amplification reagents;
3. After adding the swab, it should be stored in the refrigerator, and the main components should be tested as soon as possible.
Inactivation preservation solution:
The inactivation preservation solution fully mixes the collected sample with the virus lysis solution and the virus nucleic acid preservation solution to achieve the inactivation of the virus in the sample, while effectively ensuring the integrity of the virus nucleic acid in the sample. The collected sample can be kept at room temperature. Transportation and long-term storage under conditions. The saved viral RNA samples can be widely used in genetic testing, enzyme-linked immunoassay (ELISA), PCR testing, etc. The inactivation preservation solution has guanidine salt and non-guanidine salt.
1. Guanidine salt
(1) The virus can be instantly lysed and inactivated, reducing the risk of sampling;
(2) Viral nucleic acid can be stored stably at room temperature for 2 weeks;
(3) It can match most nucleic acid extraction reagents.
2. Non-salt
(1) The virus can be instantaneously inactivated, reducing the risk of sampling, and the virus may recover infectivity after dilution;
(2) Viral nucleic acid can be stored stably at room temperature for 2 weeks;
(3) It can match most nucleic acid extraction reagents.
3. Direct expansion preservation solution
(1) The virus can be instantaneously inactivated, reducing the risk of sampling, and the virus may recover infectivity after dilution;
(2) Viral nucleic acid can be stored stably in cold storage for 24-48 hours;
(3) It can be directly added to the nucleic acid amplification system as a template;
(4) Match most nucleic acid extraction reagents and nucleic acid release agents.
I believe that after reading the above explanation, there is more basis for choosing the correct and suitable preservation solution. Desheng is a manufacturer of virus preservation solution. It can provide two types of preservation solutions, inactivated and non-inactivated, and can be customized by customers. It can also provide disposable virus sampling tubes and nasopharyngeal swabs, etc., to provide customers with one-stop service. If necessary, please click on the official website for consultation or directly contact our professional sales staff.
