Luminol, also known as luminol, is widely used as chemiluminescent reagent in chemiluminescent immunoassay biochemical system. At room temperature, the appearance is yellow crystal or beige powder, which is a relatively stable chemical reagent. When luminol reagent is sprayed on the blood, it will oxidize with reactive oxygen species and release blue purple fluorescence, which is called luminol reaction. And how luminol luminescence detection, there are several detection methods? There are many people do not understand, this paper will introduce luminol luminescence detection method.
Adding catalyst is a commonly used method at present. In general, the chemiluminescence reaction between hydrogen peroxide and luminol is very slow, but the reaction becomes very fast after adding some catalysts.
At present, the main catalysts of luminol include some metal complexes, transition metal ions, metal complexes mainly include hemoglobin, peroxidase; transition metal ions mainly include Fe3 +, Fe2 +, Mn2 +, CR2 +, etc. Peroxides, especially horseradish peroxidase, are often used as catalysts in chemiluminescence immunoassay. Some compounds can react with peroxidase labeled antibodies, and then chemiluminescence assay with luminol can be used to determine these compounds or antibodies. Such as digoxin, hepatitis B surface antigen are detected and analyzed by this kind of method.
In addition to the acceleration of the detection by the catalyst, some inhibitors were found in the study. These organic compounds can inhibit the chemiluminescence of luminol, such as the reducing compounds containing phenolic hydroxyl group, which can react with the oxidant in the reaction process, reducing the concentration of the oxidant and reducing the chemiluminescence intensity, thus indirectly determining this kind of organic matter.
Another method is indirect determination by coupling reaction. Here, coupling reaction is to combine one reaction that can produce or consume chemiluminescence reactants with another chemiluminescence reaction, so as to realize Indirect Chemiluminescence Determination of some substances. For example, some substrates produce hydrogen peroxide under the action of some enzymes, and then produce chemiluminescence with luminol. Through the determination of chemiluminescence, we can indirectly know the purity of the substrate.
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