Native Bacillus sp. Leucine dehydrogenase
9082-71-7
2026-08-29
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Product Description
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Seller Information
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Description
ProductName Native Bacillus sp. Leucine dehydrogenase Cat No DIA-209 Description In enzymology, a leucine dehydrogenase (EC 1.4.1.9) is an enzyme that catalyzes the chemical reaction: L-leucine + H2O + NAD+ ↔ 4-methyl-2-oxopentanoate + NH3 + NADH + H+. The 3 substrates of this enzyme are L-leucine, H2O, and NAD+, whereas its 4 products are 4-methyl-2-oxopentanoate, NH3, NADH, and H+. This enzyme belongs to the family of oxidoreductases, specifically those acting on the CH-NH2 group of donors with NAD+ or NADP+ as acceptor. This enzyme participates in valine, leucine and isoleucine degradation and valine, leucine and isoleucine biosynthesis. Abbr Leucine dehydrogenase (Bacillus sp.) Source Bacillus sp. Applications This enzyme is useful for enzyme determination of L-leucine and the activity of leucine amino-peptidase. Appearance White amorphous powder, lyophilized Enzyme Commission Number EC 1.4.1.9 Activity GradeⅡ 20U/mg-solid or more (containing approx. 70% of stabilizers) CAS No 9082-71-7 Contaminants Leucylpeptide decomposing enzymes (Leu-Val) < 1.0×10⁻²% (Leu-Gly-Gly) < 1.0×10⁻²% NADH oxidase < 1.0×10⁻²% Molecular Weight 245 kDa pH Stability pH 5.5-10.5 (25°C, 20hr) Michaelis Constant 1.0×10⁻³M (L-Leucine), 3.9×10⁻⁴M (NAD⁺), 3.5×10⁻⁵M (NADH), 3.1×10⁻⁴M [α-Ketoisocaproate (α-KIC)], 2.0×10⁻¹M (NH₃) Structure 6 subunits per mol of enzyme Optimum pH 10.5-10.8 (L-Leu→α-KIC), 9.4 (α-KIC→L-Leu) Optimum temperature above 70°C Thermal stability below 60°C (pH 6.9, 10min) Stability Stable at-20°C for at least one year Stabilizers 2-Mercaptoethanol, L-cysteine, dithiothreitol, ethylenediaminetetraacetate Inhibitors Na₂S, Hg⁺⁺, Cu⁺⁺, Co⁺⁺, Mg⁺⁺, p-chloromercuribenzoate Synonyms EC 1.4.1.9; Leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); L-leucine dehydrogenase; L-leucine: NAD+ oxidoreductase (deaminating); LeuDH category Amino Acids and Derivatives(c1517) cas_num 9082-71-7 Basic Info-
Product Name:
Leucine dehydrogenase
Other Name:Leucine dehydrogenase;Dehydrogenase,leucine;E.C. 1.4.1.9;L-Leucine dehydrogenase;EC 1.4.1.9;Valine/leucine dehydrogenase;L-Leucine dehydrogenases;Valine/leucine dehydrogenases;Leucine dehydrogenases;L-Leucine dehydrogenases;L-Leucine dehydrogenase;1: PN: CN108103038 SEQID: 3 claimed sequence
CAS No.:9082-71-7
HScode:35079090
Categories:
Characteristics-
Appearance:
slightly brown solution
Storage Conditions:−20°C
Hazard IdentificationClassification of the substance or mixture
no data available
GHS label elements, including precautionary statements
Pictogram(s) no data available Signal word no data available
Hazard statement(s) no data available
Precautionary statement(s) Prevention no data available
Response no data available
Storage no data available
Disposal no data available
Other hazards which do not result in classification
no data available
Handling and StoragePrecautions for safe handling
Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.
Conditions for safe storage, including any incompatibilities
Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.
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Seller Information
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Business Type:
Manufactory
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Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Location:
Shirley, New York 11967, USA
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Payment Terms:
TT against copy of documents,D/P
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Average lead Time:
15
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Total Annual Revenue:
$1 million-$2.5 million
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Year of Establishment:
2004
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