5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
6578-06-9
2026-07-22
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Product Description
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Seller Information
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Description
ProductName 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt Cat No CSUB-0050 Applications For the colorimetric detection of alkaline phosphatase-labeled molecules, 5-Bromo-4-chloro-3-indolyl phosphate (BCIP) and Nitro Blue Tetrazolium (NBT) are available. The BCIP/NBT substrate system is versatile and functions in a variety of applications, including Northern Southern, and Western blotting, in situ hybridization, and immunohistochemistry. BCIP is provided in two salt forms: the disodium salt which is soluble in water and the p-toluidine form which is soluble in dimethylformamide. These salt forms may be used to prepare a stock solution that in combination with NBT and a reaction buffer, form a substrate solution for alkaline phosphatase. This substrate system, when incubated with alkaline phosphatase, produces an insoluble NBT diformazan product that is easily observable with its purple color. For added convenience, a mixture of BCIP and NBT is provided in an easily dissolvable tablet form or as a ready-to-use liquid. Enzyme Commission Number 229-506-1 CAS No 6578-06-9 Molecular Weight 433.62 Storage 2-8°C Synonyms BCIP p-toluidine salt; X-phosphate p-toluidine salt Molecular Formula C8H6BrClNO4P · C7H9N MDL Number MFCD00040636 PubChem Substance ID 24850179.0 Substrates Alkaline Phosphatase category Coordination Complexes(c1342) cas_num 6578-06-9 Basic Info-
Product Name:
5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
Other Name:1H-Indol-3-ol,5-bromo-4-chloro-,3-(dihydrogen phosphate),compd. with 4-methylbenzenamine (1:1);Indol-3-ol,5-bromo-4-chloro-,dihydrogen phosphate (ester),compd. with p-toluidine (1:1);1H-Indol-3-ol,5-bromo-4-chloro-,dihydrogen phosphate (ester),compd. with 4-methylbenzenamine (1:1);Indol-3-ol,5-bromo-4-chloro-,dihydrogen phosphate,compd. with p-toluidine;p-Toluidine,compd. with 5-bromo-4-chloroindol-3-ol dihydrogen phosphate (ester) (1:1);Benzenamine,4-methyl-,compd. with 5-bromo-4-chloro-1H-indol-3-yl dihydrogen phosphate (1:1);5-Bromo-4-chloro-3-indolol phosphate mono-p-toluidine salt;5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt
CAS No.:6578-06-9
Molecular Formula:C8H6BrClNO4P.C7H9N
InChIKeys:InChIKey=QEIFSLUFHRCVQL-UHFFFAOYSA-N
Molecular Weight:433.621
Exact Mass:431.964111
EC Number:229-506-1
HScode:29339190
Categories:
Characteristics-
PSA:
118.38000
XLogP3:5.21370
Appearance:crystals
Melting Point:194-195 °C
Boiling Point:580.2ºC at 760 mmHg
Flash Point:304.7ºC
Storage Conditions:2-8ºC
Hazard IdentificationClassification of the substance or mixture
Acute toxicity - Category 4, Oral
Acute toxicity - Category 4, Dermal
Acute toxicity - Category 4, Inhalation
GHS label elements, including precautionary statements
Pictogram(s) 
Signal word Warning
Hazard statement(s) H302 Harmful if swallowed
H312 Harmful in contact with skin
H332 Harmful if inhaled
Precautionary statement(s) Prevention P264 Wash ... thoroughly after handling.
P270 Do not eat, drink or smoke when using this product.
P280 Wear protective gloves/protective clothing/eye protection/face protection/hearing protection/...
P261 Avoid breathing dust/fume/gas/mist/vapours/spray.
P271 Use only outdoors or in a well-ventilated area.
Response P301+P317 IF SWALLOWED: Get medical help.
P330 Rinse mouth.
P302+P352 IF ON SKIN: Wash with plenty of water/...
P317 Get medical help.
P321 Specific treatment (see ... on this label).
P362+P364 Take off contaminated clothing and wash it before reuse.
P304+P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing.
Storage none
Disposal P501 Dispose of contents/container to an appropriate treatment and disposal facility in accordance with applicable laws and regulations, and product characteristics at time of disposal.
Other hazards which do not result in classification
no data available
Handling and StoragePrecautions for safe handling
Handling in a well ventilated place. Wear suitable protective clothing. Avoid contact with skin and eyes. Avoid formation of dust and aerosols. Use non-sparking tools. Prevent fire caused by electrostatic discharge steam.
Conditions for safe storage, including any incompatibilities
Store the container tightly closed in a dry, cool and well-ventilated place. Store apart from foodstuff containers or incompatible materials.
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Seller Information
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Business Type:
Manufactory
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Main Products:
Coenzymes,Inhibito,Enzymes,Zymogens,Substrates,Native Microorganism Creatine Amidinohydrolase
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Location:
Shirley, New York 11967, USA
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Payment Terms:
TT against copy of documents,D/P
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Average lead Time:
15
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Total Annual Revenue:
$1 million-$2.5 million
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Year of Establishment:
2004
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Country Product Purchase Quantity Date Posted Post an enquiry for this product