CAS: 42464-96-0 5-AMINO-1MQ

Name:  Lynn
Wechat：  15914422644

Tel：+86  15914422644

Whatsapp: +852 6252 8290

Email：    guangsheng 5   @zgguangsheng.com

I. Core Basic Information

II. Mechanism of Action

1. NNMT Inhibition

   
   

   It targets the substrate-binding site of NNMT, blocking its catalytic activity of transferring methyl groups from S-adenosylmethionine (SAM) to nicotinamide, reducing the production of 1-methylnicotinamide and preventing the consumption of NAD⁺ synthesis precursors.
   
   
2. NAD⁺ Level Elevation

   
   

   It restores the availability of nicotinamide, promotes NAD⁺ synthesis through the salvage pathway, enhances mitochondrial function and SIRT1 activity, and improves metabolic and energy efficiency.
   
   
3. Metabolic Remodeling

   
   

   It reduces adipogenesis and lipid droplet accumulation, enhances insulin sensitivity, lowers plasma cholesterol and blood glucose levels, preserves muscle mass, and boosts mitochondrial ATP production.
   
   
4. Tissue Specificity

   
   

   It preferentially acts on tissues with high NNMT expression, such as adipose tissue, liver, and muscle, without exerting significant effects on related methyltransferases and the NAD⁺ salvage pathway.
   
   

III. Core Biological Activities & Research Value

IV. Research Applications & Therapeutic Potential

1. Research Tool

   
   

   It is used for studying NNMT pathway mechanisms, metabolic remodeling, mitochondrial function, and SIRT1-related signaling. It is applicable to mouse models of obesity, insulin resistance, muscle injury, etc. (e.g., 20 mg/kg/d subcutaneous administration is effective in DIO models).
   
   
2. Therapeutic Potential Directions
   
   
   • Metabolic Diseases: Type 2 diabetes, obesity, non-alcoholic fatty liver disease.
   • Muscle & Metabolism: Muscle atrophy, exercise recovery, metabolic syndrome.
   • Cancer Adjuvant Therapy: Inhibition of metastasis in NNMT-highly expressed tumors.
   • Neurodegenerative Diseases: Exerting effects through energy metabolism regulation and neuroprotection.
   
   

V. Usage & Safety Guidelines

1. Reconstitution

   
   

   Dissolve in sterile water or PBS to prepare a stock solution of 1–10 mg/mL. Prepare fresh for immediate use; aliquot and freeze the remaining solution for storage.
   
   
2. Administration Route

   
   

   Common routes in mice include subcutaneous injection (20 mg/kg/d), intraperitoneal injection, or oral administration. Dosage and frequency should be adjusted according to specific animal models.
   
   
3. Safety Profile

   
   

   No obvious toxicity was observed in preclinical studies with mice at a dose of 60 mg/kg/d. For research use only; not approved by the FDA for human use. Avoid contact with skin and mucous membranes; wear appropriate personal protective equipment (PPE) during operation.
   
   
4. Compliance Reminder

   
   

   Not for sale as a dietary supplement or pharmaceutical product. Athletes should comply with WADA's ban on unapproved substances.