Free Sample Non GMO Natto Extract Nattokinase Enzyme Supplement for Blood Pressure

Product Name: Nattokinase
CAS Number: 9014-01-1
Molecular Weight: Approximately 27.7 kDa (27,700 Da)
Appearance: White to off-white powder

Nattokinase is a serine protease enzyme originally isolated from natto, a traditional Japanese fermented soybean food produced by Bacillus subtilis natto. It is a single-chain polypeptide composed of approximately 275 amino acid residues, with a molecular mass of about 27.7 kDa. The enzyme is typically obtained as a white to off-white lyophilized powder. It is soluble in water and aqueous buffers, forming clear to slightly opalescent solutions, and is practically insoluble in most organic solvents. Nattokinase exhibits optimal proteolytic activity under mildly alkaline conditions, generally around pH 8.0, and is active across a broad pH range. It is thermolabile and may lose activity upon prolonged exposure to elevated temperatures, typically above 50°C. As a serine protease, it catalyzes the hydrolysis of peptide bonds and demonstrates fibrinolytic activity by degrading fibrin and related proteins. It is used in dietary supplements, functional foods, and biochemical research. The material should be stored in a tightly closed container in a cool, dry, dark place, preferably refrigerated or frozen, to maintain enzymatic stability. It should be protected from moisture, heat, and strong oxidizing agents.

For regulatory compliance (e.g., USP, EP, JP), raw material Vidarabine must meet several rigorous standards:

• Assay (by HPLC): 98.0% – 102.0% on an anhydrous basis.

• Related Substances: Limits for specific impurities, notably Ara-Hx (NMT 2.0%), as well as any unspecified individual impurity (NMT 0.1%) and total impurities (NMT 2.0%).

• Residual Solvents: Must comply with ICH Q3C guidelines (Class 1 and 2 solvents limited or absent).

• Heavy Metals: NMT 20 ppm (typically lower for injectable grades).

• Water Content (Karl Fischer): NMT 6.0% (the material is hygroscopic to a degree).

• Microbial Limits: Total aerobic microbial count (TAMC) ≤ 1000 CFU/g, absence of S. aureus, P. aeruginosa, and Candida albicans.