Thymosin β4 acetate1346423-89-9

1. Basic Chemical Information

1. Full Name: Thymosin Beta 4 Acetate
2. Abbreviation: Tβ4, TB500
3. Amino Acid Sequence (43 amino acids, N-terminal acetylated):
   
   
   Ac-Ser-Asp-Lys-Pro-Asp-Met-Ala-Glu-Ile-Glu-Lys-Phe-Asp-Lys-Ser-Lys-Leu-Lys-Lys-Thr-Glu-Thr-Gln-Glu-Lys-Asn-Pro-Leu-Pro-Ser-Lys-Glu-Thr-Ile-Glu-Gln-Glu-Lys-Gln-Ala-Gly-Glu-Ser-OH
4. Molecular Formula: \(\mathrm{C_{212}H_{350}N_{56}O_{78}S}\)
5. Molecular Weight: 4963.44 Da (free base); approx 5023.5 Da (acetate salt)
6. Isoelectric Point (pI): 5.1
   
   
   It carries net negative charge under physiological pH 7.4.

2. Physical Properties

1. Appearance: White to off-white loose lyophilized powder or lyophilized cake, fluffy honeycomb texture, light and easy to fly when touched.
2. Odor: Almost odorless.
3. Solubility: Freely soluble in pure water, aqueous buffer solution; completely clear solution without insoluble residues after dissolution.
4. Storage Condition: Sealed, protected from light, stored at -20°C for long-term preservation; avoid repeated freeze-thaw cycles.

3. Chemical Stability

1. Stable under dry low-temperature environment; easy to degrade when exposed to high temperature, moisture or strong light.
2. Sensitive to strong acid and strong alkaline solution; long-term placement in extreme pH will cause peptide chain hydrolysis.
3. No obvious oxidation under sealed inert gas protection; susceptible to oxidation if exposed to air for a long time.

4. Biological Characteristics

1. Promotes angiogenesis, accelerates wound tissue repair and skin regeneration.
2. Regulates cell migration, reduces inflammatory response.
3. Improves tissue recovery of muscle, tendon and joint injury.
4. For laboratory research use only, not approved for human clinical medication worldwide.

5. Technical Quality Indicators (Standard API Grade)

1. HPLC Purity: ≥98.0%
2. Water Content: ≤8.0%
3. Residual Trifluoroacetic Acid (TFA): ≤0.5%
4. Single Maximum Impurity: ≤1.0%
5. Heavy Metals (Pb, As, Cd, Hg): Meet research-grade limit standards
6. Endotoxin: ≤10 EU/mg