High Purity Erythroid Cell Research Freeze-Dried Lab Culture Reagent
Brief Overview
This glycosylated cell signal lyophilized material is produced via recombinant mammalian cell expression technology, exclusively for cell cultivation, basic hematology trials, renal cell tests and oxygen metabolism related fundamental lab research from university laboratories, biomedical research institutes and biotechnology R&D teams. Adopting low-temperature vacuum freeze-drying craft, the finished uniform off-white powder maintains stable cell receptor binding performance and highly repeatable experimental data during long-term cell strain passaging and tissue slice assays. We provide 3 kinds of vacuum-sealed neutral glass vial specs for different lab plans: 100μg, 500μg, 1mg. Each batch comes with complete technical archives including exclusive batch inspection reports and unified technical datasheets, only for laboratory internal filing and academic reference.
Core Laboratory Research Application & Functional Characteristics
1. Usage Restriction Statement
This cell signal solid is strictly limited to laboratory-only basic cell research. It must not be used for human oral intake, external application, physical conditioning or animal disease intervention of any kind. All authorized research directions are listed below:
- Quantitative detection of cell receptor binding affinity in vitro with erythroid progenitor cell culture systems
- Comparative cell model studies on the basic mechanism of erythroid cell proliferation, differentiation and maturation
- Observation trials of renal cell endocrine secretion and intracellular oxygen-sensing signal pathways
- Fundamental exploration of hypoxia-triggered transcription factor regulation and hematopoietic cell metabolism
- Biochemical research on directional cultivation of hematopoietic stem cells and bone marrow microenvironment
- Gradient comparison tests of erythroid precursor proliferation efficiency under different additive concentrations
- Lab verification of the correlation between molecular glycosylation structure and solution stability
- Exploration of oxidative stress balance and intracellular iron signal linkage in red blood precursor cells
- Long-period cell cultivation tests of hematopoietic cell damage repair and intracellular endocrine feedback regulation
2. Core Laboratory Research Advantages
Specific Cell Surface Receptor Binding Feature
The complete glycosylated molecular structure can specifically recognize and bind receptors on erythroid precursor cell surfaces, steadily activate downstream intracellular differentiation signal cascades in test systems. It supplies low-interference, single-variable comparison data for hematology signal research, effectively eliminating confounding factors in cell culture control groups.
Long-Term Stability After Freeze-Drying
Low-temperature vacuum dehydration locks intact protein backbones and natural glycosylated side chains, preventing molecular degradation, aggregation and desialylation during storage and cross-border transport. It solves the activity fading issue of liquid samples, cutting repeated preparation workload for long-term lab inventory and continuous cell passaging work.
Broad Compatibility With Common Mammalian Cell Culture Systems
This powder dissolves fully in lab bacteriostatic purified water and neutral pH-adjusted PBS buffer. It adapts to conventional hematopoietic stem cells, TF-1 cell lines, renal tubular epithelial cells and bone marrow slice test systems, without introducing exogenous contaminants that disrupt cell growth microenvironment.
Regular Concentration-Dependent Gradient Responses
Adjustable working concentrations fit customized experimental designs; indicators including erythroid proliferation rate, intracellular hemoglobin production and hypoxia responses show steady linear changes with different additive dosages, convenient for quantitative statistics and academic paper charting in basic hematology studies.
Ultra-Low Detection Background Interference
Multi-stage layered chromatographic purification minimizes residual host cell protein fragments, incomplete glycosylation byproducts, polymers and endotoxin impurities. It produces no extra noise in spectrophotometry, fluorescence assays and cell proliferation colorimetric tests, ensuring reliable, clear biochemical quantitative results.
Consistent Batch Performance
Integrated workflows of standardized recombinant expression, multi-step chromatographic purification and vacuum freeze-drying guarantee identical purity, dissolution uniformity and receptor binding performance across all batches. It avoids result deviations from inconsistent raw material lots, supporting parallel control trials and repeated experimental validation.
Complete Standard Physical & Chemical Specification Parameters
Basic Product Identification Information
- Full Standard Product Name: Erythroid Cell Signal Glycoprotein Laboratory Culture Solid Reagent
- Alias: Renal Cell Oxygen-Sensing Hematology Research Powder
- Amino Acid Sequence: 165 amino acid residues of standard native cell-derived sequence
- Core Glycosylated Polypeptide Formula: C₈₀₉H₁₃₀₀N₂₂₉O₂₄₆S₅ (glycan side chains follow standard modification ranges)
- Theoretical Core Molecular Weight: 18.3 kDa; Apparent Glycosylated Molecular Weight: 36–40 kDa
- Isoelectric Point Range: 4.2–5.2
- Appearance: Uniform off-white freeze-dried powder, no caking, discoloration, brown spots, foreign particles or insoluble visible impurities
- Odor: Odorless
- Solubility: Fully miscible with lab bacteriostatic purified water and neutral PBS buffer; insoluble in alcohol-based organic solvents, aqueous solubility ≥1mg/mL
- Reconstituted Liquid Appearance: Pale straw clear transparent solution with no turbidity, flocculation, precipitate or suspended particles after gentle full dissolution
- pH (0.5mg/mL aqueous solution): 6.0 ~ 7.2
- Residual Moisture: ≤ 2.0% (Karl Fischer titration per batch)
- Endotoxin Level: < 0.1 EU/mg, meeting global biosafety criteria for cell-based lab research
- Heavy Metal Limits: Lead ≤10ppm, Arsenic ≤2ppm, Cadmium ≤1ppm, Mercury ≤1ppm
- Microbial Criteria: Total aerobic bacteria <10 CFU/mg, mold & yeast <5 CFU/mg; no pathogenic microbes detected in all batches
- Structural Integrity: Every batch undergoes spectrum matching, peptide mapping and mass spectrometry to confirm intact amino acid chains, complete disulfide bonds and uniform glycosylation, with no chain breakage or desialylation defects
- Cell Activity Standard: ≥150,000 activity units/mg, validated via TF-1 cell proliferation quantitative assays
Available Sealed Vial Sizes (No bulk kilogram supply)
- EPO-0100: 100μg freeze-dried erythroid cell signal powder per vacuum-sealed neutral borosilicate glass vial
- EPO-0500: 500μg freeze-dried erythroid cell signal powder per vacuum-sealed neutral borosilicate glass vial
- EPO-1000: 1mg freeze-dried erythroid cell signal powder per vacuum-sealed neutral borosilicate glass vial
Packaging Configuration
- Vial: Lab-grade neutral borosilicate glass, airtight inert butyl rubber stopper, full aluminum crimp vacuum seal for long-term moisture & light protection
- Single-unit outer package: Independent shockproof foam tray + moisture-proof light-blocking aluminum foil bag; bulk orders use thick reinforced corrugated cartons with shock-absorbing fillers
- MOQ: 1 vial for lab trial testing; 10 vials for regular institutional bulk procurement
- Trial option: 100μg test vials available for pre-purchase lab validation before large orders
- Custom service: Custom printed batch labels and institute branding can be arranged for institutional bulk orders with prior written notice
Production & QC Standards
All products are manufactured and tested following international biological lab reagent norms. Each batch undergoes full multi-index testing, with signed exclusive batch reports delivered with every order. Test procedures comply with USP <1231> biological reagent guidelines, ICH Q3A impurity rules and ISO 17034 analytical lab standards.
Core Purity Requirement
Finished powder chromatographic purity ≥98.0% verified by SEC-HPLC and SDS-PAGE dual tests; full chromatogram and electrophoresis records attached to each batch report. Single unknown impurity ≤0.3%, total unknown impurities ≤1.0%. C4 reversed-phase and size-exclusion columns with 280nm UV detection are used to measure purity, glycosylation uniformity and polymer residues.
Mandatory Batch Test Items
- Mass spectrometry molecular weight matching, deviation controlled within ±100 Da of theoretical glycosylated values
- Full peptide mapping validation to match standard native molecular configuration
- Trace host cell protein and production reagent testing per ICH Q3C limits
- Constant temperature-humidity accelerated stability tests for shelf-life confirmation
- Quantitative assay of receptor binding and TF-1 cell proliferation activity consistency
- 100% manual visual inspection of all sealed vials pre-shipment to reject flawed units
- Full batch traceability covering cell culture logs, production records, full test data and delivery archives
Reconstitution Operation Guide
Recommended Solvent: Lab bacteriostatic purified water / pH 6.8–7.2 PBS buffer
- Slowly add solvent along the vial inner wall to avoid foaming and protein structural damage
- Fully tighten the cap, swirl gently in circular motions for 3–5 minutes until all powder dissolves completely
- Do not shake vigorously; harsh agitation causes protein aggregation, broken disulfide bonds and weakened receptor binding capacity
Working Concentration Range
0.1ng/mL – 100ng/mL for erythroid & renal cell model research, adjustable based on custom experimental design, cell strains and test endpoints
Storage Rules
Unopened Vacuum-Sealed Vials
- Long-term storage: -20℃ ~ -80℃, fully protected from direct sunlight and high humidity
- Valid shelf life at stable -20℃: 12 months
- Valid shelf life at stable -80℃: 36 months
- Transit stability: Retain full activity for up to 7 days at ambient temperature ≤25℃
- Forbidden practices: Frequent temperature cycling, prolonged sun exposure, storage adjacent to volatile organic reagents
Reconstituted Liquid
- Storage: 2℃ ~ 8℃ refrigeration, light-proof
- Usage deadline: Consume all liquid within 14 days after preparation
- Strict ban: Do not freeze diluted liquid; repeated freeze-thaw cycles permanently damage protein structure and research-related activity
Shipping Guidelines
- Small trial orders can use standard international express for short cross-border routes
- Large batch shipments are advised to use insulated foam packaging with cold packs to stabilize molecular structure during long overseas transit
- Full supporting documents (technical datasheet, batch COA, structural verification files) are included in every parcel for customs checks and lab archiving
Lab Operation Safety Rules
- Wear disposable nitrile gloves, impact-resistant safety goggles and dust-proof lab masks during powder weighing and liquid preparation
- Prevent direct skin/mucosa contact and inhalation of dry lyophilized powder
- In case of accidental exposure, flush the affected area with abundant clean running water continuously for no less than 5 minutes
- Dispose of leftover powder and waste liquid strictly following your institute’s biosafety waste regulations; never discharge waste into domestic drains or general household trash
Usage Limitation & Disclaimer
This cell signal glycoprotein powder is solely developed for fundamental scientific research of universities, biomedical institutes and biotechnology R&D teams. It is not a medical supply, therapeutic raw material or cosmetic ingredient, and has not obtained global regulatory authorization for any human or animal intervention use. Resale to private individuals for personal body adjustment or unregulated self-use is strictly prohibited. Any human-related experimental trials can only proceed after obtaining formal written regulatory approval.
Free Post-Shipment Technical Documents
- Batch-specific COA report with complete HPLC/SEC-HPLC raw spectrum data
- Full official technical datasheet covering all specs, reconstitution steps and storage protocols
- Molecular structure diagram, peptide mapping and glycosylation validation files
- Full test records for heavy metals, endotoxin, microbes and host cell proteins
- Original accelerated stability test long-term data
- Custom lab operation guides available upon written customer request for internal archiving and biosafety training
