High PuritHigh Purity FOXO4-DRI Cell Signal Freeze-Dried Lab Research Solid Reagenty FOXO4-DRI Cell Signal Freeze-Dried Lab Research Solid Reagent

Brief Overview FOXO4-DRI, full name D-Retro-Inverso FOXO4 synthetic peptide, is a cell-permeable polypeptide signal reagent produced via standard solid-phase D-amino acid peptide synthesis technology, only supplied for FOXO4-p53 protein binding interaction research, senescent cell in-vitro culture model construction, transcription factor signal pathway analysis, cartilage cell metabolic mechanism detection and cell apoptosis basic laboratory research carried out by university labs, biomedical research institutes and biotechnology R&D teams. Adopting low-temperature vacuum lyophilization process, the finished uniform off-white lyophilized powder maintains stable intracellular peptide penetration performance and highly repeatable cell model test data during long-term cell strain passaging and multi-tissue slice in-vitro culture experiments. Independent vacuum-sealed neutral borosilicate glass vial specifications are provided to match diversified laboratory test schemes: 1mg,5mg,10mg. Each production batch is equipped with complete technical archives including exclusive batch inspection reports and unified technical datasheets, only for internal laboratory filing and academic reference.

Core Laboratory Research Application & Functional Characteristics

Superior Long-Term Intracellular Stability Brought by D-Amino Acid Modification Low-temperature vacuum dehydration locks intact complete D-amino acid polypeptide backbone and uniform retro-inverso structural arrangement, preventing molecular degradation, fragment dissociation and non-specific aggregation during storage and cross-border transportation. D-type amino acid modification effectively avoids degradation by intracellular proteases, solves the problem of rapid loss of signal activity of natural L-peptide liquid medium samples, greatly reduces repeated reagent preparation workload for long-term laboratory inventory management and continuous cell passaging experiments, and supports multi-day continuous cell culture test schemes without frequent medium replenishment.

Broad Compatibility With Common Mammalian Cell Culture Systems This freeze-dried powder can be fully dissolved in laboratory bacteriostatic purified water and neutral pH-adjusted PBS buffer solution. It is compatible with conventional senescent fibroblasts, cartilage precursor cells, mesenchymal stem cells, testicular interstitial cells and multi-tissue slice test systems, will not introduce exogenous contaminants that disrupt the cell growth microenvironment, and will not cause abnormal cell toxic reactions within the standard research concentration range.

Regular Concentration-Dependent Gradient Responses Adjustable working concentrations adapt to customized experimental designs; indicators such as senescent cell apoptosis ratio, intracellular senescence marker protein synthesis and p53 signal molecule expression levels show stable linear changes under different additive dosages, which is convenient for quantitative statistics and academic paper chart drawing in basic transcription factor signal research.

Ultra-Low Detection Background Interference Multi-stage layered reversed-phase chromatographic purification minimizes residual truncated polypeptide fragments, incomplete D-amino acid modification by-products, aggregate impurities and endotoxin contaminants in finished solids. It will not generate additional noise signals in spectrophotometers, fluorescence detectors and cell proliferation colorimetric testing equipment, ensuring reliable and clear biochemical quantitative detection results and stable repeatability of cell signal verification experiments.

Consistent Batch Performance Across All Production Lots Integrated process of standardized solid-phase D-peptide synthesis, multi-step chromatographic purification and vacuum freeze-drying ensures consistent polypeptide purity, dissolution uniformity and FOXO4-p53 binding blocking signal performance of all batches. It avoids experimental result deviations caused by inconsistent raw material synthesis batches, and supports parallel control experiments and repeated result verification for aging signal laboratory research teams.

Complete Standard Physical & Chemical Specification Parameters Basic Product Identification Information Full Standard Product Name: FOXO4-DRI D-Retro-Inverso Transcription Factor Blocking Polypeptide Laboratory Culture Solid Reagent Alias: FOXO4-p53 Interaction Interference Peptide Senescent Cell Laboratory Research Lyophilized Powder Peptide Sequence: Full D-amino acid modified 34-residue retro-inverso standard sequence Core Polypeptide Molecular Formula: C₂₂₈H₃₈₈N₈₆O₆₄ Theoretical Total Molecular Weight: 5358.05 Da Isoelectric Point Range: 10.2–10.8 Appearance Morphology: Uniform white to off-white freeze-dried solid powder, no caking, discoloration, yellow/brown spots, tiny foreign particles or visible insoluble impurities Odor Feature: Completely odorless Solubility Characteristic: Fully miscible with laboratory bacteriostatic purified water and neutral PBS buffer liquid; insoluble in alcohol-based organic liquid medium, aqueous buffer solubility ≥2mg/mL Reconstituted Liquid Appearance: Pale colorless clear transparent solution with no turbidity, flocculation, precipitate or suspended particles after gentle full dissolution pH Value of Standard 1mg/mL Aqueous Mixing Solution: 7.0 ~ 7.8 Residual Moisture Index: ≤ 2.0% (tested by Karl Fischer titration method for each independent batch) Endotoxin Level: < 0.1 EU/mg, fully meet global universal biosafety standards for cell-based laboratory basic research Total Heavy Metal Impurity Limit Standard: Integrated total heavy metal contaminants ≤10ppm (comprehensive combined detection of multiple heavy metal elements without separate single element labeling) Microbiological Control Standard: Total aerobic bacteria <10 CFU/mg, mold & yeast <5 CFU/mg; no pathogenic microorganisms detected in all finished production batches Molecular Structural Integrity Standard: Every batch undergoes spectrum matching, peptide mapping and mass spectrometry verification to confirm intact full D-amino acid polypeptide chain, complete retro-inverso structural arrangement, no chain breakage or fragment dissociation defects Standard Cell Signal Activity Benchmark: FOXO4-p53 binding blocking biological activity ≥99% relative reference standard, validated via senescent fibroblast signal quantitative detection assay

Available Sealed Vial Sizes (No bulk kilogram raw material supply) FOXO4-001: 1mg freeze-dried transcription factor signal solid per vacuum-sealed neutral borosilicate glass vial FOXO4-005: 5mg freeze-dried transcription factor signal solid per vacuum-sealed neutral borosilicate glass vial FOXO4-010: 10mg freeze-dried transcription factor signal solid per vacuum-sealed neutral borosilicate glass vial

Packaging Configuration Details Vial Material: Lab-grade neutral borosilicate glass, airtight inert butyl rubber stopper, full aluminum crimp vacuum seal for long-term moisture & light protection (synthetic polypeptide has slight hygroscopicity, complete vacuum sealing is required to retain stable intracellular penetration signal activity) Single-unit outer protective package: Independent shockproof foam tray + moisture-proof light-blocking aluminum foil sealed bag; multi-vial bulk institutional orders use thick reinforced corrugated cartons filled with shock-absorbing filler materials Minimum Order Standard: 1 vial for preliminary laboratory trial test verification; 10 vials for regular small-batch institutional laboratory procurement Trial Sample Support: 1mg test vials available for pre-purchase lab effect verification before large-volume batch procurement Customized Service Item: Custom printed exclusive batch labels and institutional laboratory brand marking can be arranged for bulk institutional orders with advance written notification

Strict Production & Batch Inspection Quality Control Standards All FOXO4-DRI transcription factor signal solid reagents are manufactured and tested following unified international biological laboratory peptide reagent testing specifications. Each independent production batch completes full multi-index comprehensive testing, with signed official exclusive batch inspection reports delivered alongside every customer order. All inspection operating procedures comply with USP <1231> biological reagent testing guidelines, ICH Q3A impurity control rules and ISO 17034 analytical laboratory quality standards.

Core High Purity Benchmark Finished D-amino acid polypeptide solid chromatographic purity ≥99.0% verified by reversed-phase HPLC dual detection method; complete chromatogram imaging records are attached to each batch test report. Single unknown individual impurity ≤0.3%, total sum of all unknown impurities ≤1.0%. C18 reversed-phase chromatographic column with 220nm UV wavelength detection are adopted for accurate purity value measurement, simultaneously verifying D-amino acid modification uniformity and dissociated peptide fragment aggregate residual content.

Mandatory Full Batch Inspection Items Mass spectrometry molecular weight matching test, deviation between measured full peptide molecular weight and theoretical standard value controlled within ±150 Da Full peptide mapping structural validation to confirm full matching with standard native FOXO4-DRI D-amino acid molecular configuration Trace residual peptide synthesis auxiliary reagent and intermediate impurity testing complying with ICH Q3C standardized residual limit requirements Constant temperature-humidity accelerated stability test for shelf-life validity confirmation Quantitative consistency detection of FOXO4-p53 binding blocking and senescent cell signal regulation biological activity 100% manual visual full inspection of all sealed finished vials before shipment to screen defective units with abnormal appearance or incomplete vacuum sealing Complete batch full traceability management system covering solid-phase peptide synthesis raw material logs, synthesis production operation records, full-item inspection data and outgoing delivery archive files

Standard Step-by-Step Dissolving Operation Guidance Recommended Matching Solvent Medium: Laboratory dedicated bacteriostatic purified water / pH 7.0–7.8 neutral PBS buffer Slowly inject prepared buffer medium along the inner wall of the glass vial to avoid violent foaming and polypeptide structural denaturation or fragment dissociation Fully tighten the vial cap, rotate and swirl gently in slow circular motions for 3–5 minutes until all freeze-dried solid powder dissolves completely (synthetic polypeptide has mild hygroscopicity, slow gentle dissolution is required to avoid incomplete solvation and peptide separation) DO NOT shake the vial violently; harsh agitation will trigger peptide fragment dissociation, structural rearrangement and weakened stable FOXO4-p53 binding blocking signal activity

Suggested Working Concentration Range For Senescence & Transcription Factor Cell Model Basic Research 10nM – 200nM, adjustable flexibly based on customized experimental design, cell strain varieties and test endpoint detection indicators

Storage Environment & Valid Usage Cycle Regulatory Specifications Unopened Original Vacuum-Sealed Vials Long-term sealed storage environmental condition: Store fully sealed vials at -20℃ ~ -80℃, completely shielded from direct sunlight and high ambient humidity (hygroscopic synthetic polypeptide must avoid repeated exposure to humid air) Valid stable storage duration under constant -20℃ freezing environment: 12 months Valid stable storage duration under constant -80℃ ultra-low temperature environment: 36 months Short-distance transportation stability tolerance: Retain complete transcription factor binding blocking signal activity for up to 7 days under ambient temperature ≤25℃ Forbidden storage operation behaviors: Frequent repeated temperature cycling fluctuation, long-time continuous direct sunlight exposure, storage adjacent to volatile organic chemical reagents, repeated opening of sealed vial in humid atmospheric environment

Liquid Mixed With Bacteriostatic Purified Water / PBS Buffer After Full Dissolution Storage condition after complete dissolution: Place inside refrigerator under 2℃ ~ 8℃ refrigeration environment, fully shaded from light Suggested full consumption time limit: Use up all mixed liquid within 14 days after preparation completion Strict prohibition rule: Do not freeze diluted mixed liquid; repeated freeze-thaw cycles will permanently damage D-amino acid polypeptide skeleton and retro-inverso structural stability, completely lose senescent cell culture research signal regulation activity

Shipping & Receiving Handling Reminders Small trial vial orders can be delivered via standard international express for short-distance cross-border transportation routes Large multi-vial batch orders are strongly recommended to adopt temperature-insulated foam protective packaging equipped with ice packs to maintain stable D-modified polypeptide molecular structural state during long-distance overseas transportation Complete supporting shipping documents will be delivered together with all parcels, including unified technical data sheets, batch exclusive COA test records and molecular structural verification files, for customs inspection and internal laboratory filing use

Laboratory Operation Safety Precautions Wear disposable nitrile protective gloves, transparent impact-resistant safety goggles and dust-proof laboratory masks during solid powder weighing and liquid dissolving operation processes Avoid direct skin contact, eye mucous membrane contact and inhalation of dry freeze-dried transcription factor signal solid powder In case of accidental contact with skin or eye tissue, immediately flush the affected area thoroughly with abundant clean running water continuously for no less than 5 minutes Dispose residual solid powder and waste mixed liquid strictly following the internal biosafety waste management regulations of your laboratory; never pour waste liquid directly into domestic drainage pipelines or ordinary household garbage bins

Important Usage Restriction & Disclaimer Statement This FOXO4-DRI D-amino acid polypeptide transcription factor blocking solid reagent is solely developed for fundamental scientific research projects of universities, biomedical research institutes and biotechnology R&D teams. This product is not medical treatment supplies, biological therapeutic raw materials or cosmetic ingredient raw materials, and has not obtained official regulatory authorization from global supervision authorities for any human body intervention use, oral intake or animal disease diagnosis and treatment. Resale to individual private users for personal physical conditioning or unregulated self-use is strictly prohibited. Any human-related experimental trial research can only be carried out after obtaining formal written regulatory approval documents.

Free Supporting Technical Documents Provided Upon All Shipment Order Completion After order shipment completion, all customers can receive a complete set of free full technical archive files: Batch exclusive Certificate of Analysis (COA) report attached with full reversed-phase HPLC spectrum purity test raw data records Full-version unified technical data sheet covering all product specifications, standard dissolving operation steps and standardized storage regulatory rules Complete molecular structure schematic diagram, peptide mapping and D-amino acid modification structural verification file Full set inspection records of total heavy metal impurities, endotoxin, microorganisms and synthesis residual auxiliary reagent test indexes Original raw data of long-term molecular stability accelerated testing experiments Customized laboratory dedicated experiment operation guidance documents available upon customer written request, for internal institutional research file sorting and laboratory biosafety training use