High Purity HCG Glycoprotein Hormone Freeze-Dried Lab Research Solid Reagent

Brief Overview HCG is a heterodimeric glycosylated glycoprotein reagent manufactured through eukaryotic cell recombinant expression and multi-stage chromatographic purification techniques. It is exclusively prepared for academic laboratory fundamental research projects, including glycoprotein-receptor binding interaction testing, gonadal cell steroid metabolic pathway analysis, reproductive endocrine signal cascade exploration, trophoblast cell in-vitro culture model construction and multi-tissue slice co-culture biochemical mechanism research for university labs, biomedical research institutions and biotechnology R&D teams. The finished product adopts low-temperature vacuum freeze-drying production craft, presenting uniform off-white solid powder, which can maintain stable receptor binding activity and highly repeatable cell culture experimental data during long-term cell strain passaging and multi-organ tissue slice cultivation. Two independent vacuum-sealed neutral borosilicate glass vial specifications are available to match diverse laboratory testing plans: 1mg and 5mg. Each production batch is equipped with complete technical documents including exclusive batch inspection reports and unified technical datasheets, only for internal laboratory filing and academic reference use.

Core Laboratory Research Application & Functional Characteristics

Superior Long-Term Structural Stability After Lyophilization Low-temperature vacuum dehydration locks complete glycoprotein heterodimer skeleton and uniform glycosylation modification sites, preventing subunit separation, polypeptide chain degradation and non-specific molecular aggregation during storage and cross-border transportation. The freeze-dried solid solves the defect of rapid loss of receptor binding activity for liquid medium samples, largely lowers repeated reagent preparation workload for long-term laboratory inventory management and continuous cell passaging testing, and supports multi-day uninterrupted cell culture experimental schemes without frequent medium replenishment.

Broad Compatibility With Common Mammalian Cell Culture Systems This freeze-dried powder can be fully dissolved in laboratory dedicated bacteriostatic purified water and neutral PBS buffer solution with adjusted pH value. It matches conventional granulosa cells, Leydig cells, trophoblast cells, endometrial stromal cells and multi-tissue slice testing systems, will not introduce foreign contaminants that disturb cell growth microenvironment, and will not trigger abnormal cytotoxic responses within standard research concentration ranges.

Regular Concentration-Dependent Gradient Responses Adjustable working concentrations fit customized experimental designs; multiple detection indicators including steroid synthetase expression level, intracellular secondary messenger concentration and cell proliferation ratio show stable linear changes along concentration gradients, facilitating quantitative data statistics and academic paper graph drafting for basic glycoprotein signal research.

Ultra-Low Detection Background Interference Multi-stage layered reversed-phase chromatographic purification removes residual split subunit fragments, incomplete glycosylation by-products, protein aggregate impurities and endotoxin contaminants inside finished solid products. It will not generate extra noise signals for spectrophotometers, fluorescence detectors and cell proliferation colorimetric testing equipment, delivering reliable, clear biochemical quantitative detection results and stable repeatability for cell signal verification testing.

Consistent Batch Performance Across All Production Lots Integrated standardized production flow including eukaryotic cell recombinant expression, multi-step chromatographic purification and vacuum freeze-drying guarantees unified glycoprotein purity, glycosylation uniformity and receptor binding signal regulatory performance for all batches. It avoids experimental result deviation caused by inconsistent raw material expression batches, supporting parallel control testing and repeated result validation for laboratory teams researching reproductive endocrine cell signals.

Complete Standard Physical & Chemical Specification Parameters Basic Product Identification Information Full Standard Product Name: HCG Glycoprotein Reproductive Endocrine Signal Laboratory Culture Solid Reagent Alias: HCG Heterodimer Glycoprotein Receptor Binding Cell Metabolism Laboratory Research Lyophilized Powder Protein Structure: Heterodimer glycoprotein composed of shared alpha subunit and exclusive beta subunit, equipped with complete glycosylation modification on polypeptide chain Core Glycoprotein Molecular Formula: C₁₁₀₅H₁₇₇₀N₃₁₈O₃₃₆S₂₆ Theoretical Total Molecular Weight: 25.7 kDa Isoelectric Point Range: 4.8–5.4 Appearance Morphology: Uniform off-white freeze-dried solid powder, free of caking, discoloration, yellow/brown oxidation spots, tiny foreign particles or visible insoluble impurities Odor Feature: Fully odorless Solubility Characteristic: Completely miscible with laboratory bacteriostatic purified water and neutral PBS buffer liquid; insoluble in alcohol-based organic solvents, aqueous buffer solubility ≥10mg/mL Reconstituted Liquid Appearance: Transparent colorless clear liquid after full gentle dissolution, without turbidity, floccules, sediment or suspended particles pH Value of Standard 1mg/mL aqueous mixed solution: 7.0 ~ 7.6 Residual Moisture Index: ≤ 2.0% (tested via Karl Fischer titration for each separate batch) Endotoxin Level: < 0.1 EU/mg, fully complying with global universal biosafety standards for in-vitro cell basic laboratory research Total Heavy Metal Impurity Limit Standard: Comprehensive total heavy metal pollutants ≤10ppm (integrated combined detection of various heavy metal elements, no separate marking of single metal elements) Microbiological Control Standard: Total aerobic bacteria <10 CFU/mg, mold & yeast <5 CFU/mg; no pathogenic microorganisms detected in all finished batches Molecular Structural Integrity Standard: Every batch undergoes spectrum matching, peptide mapping and mass spectrometry verification to confirm intact full heterodimer subunit binding and complete glycosylation modification sites, without subunit separation or polypeptide chain fracture defects Standard Cell Signal Activity Benchmark: LHCGR receptor binding biological activity ≥98% compared with reference standard, verified through quantitative detection testing of granulosa cell steroid synthesis

Available Sealed Vial Sizes (No bulk kilogram raw material supply) HCG-001: 1mg freeze-dried glycoprotein cell signal solid per vacuum-sealed neutral borosilicate glass vial HCG-005: 5mg freeze-dried glycoprotein cell signal solid per vacuum-sealed neutral borosilicate glass vial

Packaging Configuration Details Vial Material: Laboratory grade neutral borosilicate glass, airtight inert butyl rubber stopper, full aluminum crimp vacuum sealing for long-term moisture and light shielding protection (glycosylated glycoprotein carries slight hygroscopicity and photosensitivity, full vacuum light-proof sealing is required to retain stable receptor binding signal activity) Single-unit outer protective package: Independent shockproof foam tray + moisture-proof light-blocking aluminum foil sealed bag; multi-vial institutional bulk orders adopt thick reinforced corrugated cartons filled with shock-absorbing filling materials Minimum Order Standard: 1 vial for preliminary laboratory trial testing verification; 10 vials for regular small-batch institutional laboratory procurement Trial Sample Support: 1mg test vials available for pre-purchase laboratory effect verification before large-volume batch procurement Customized Service Item: Custom printed exclusive batch labels and institutional laboratory brand marking can be arranged for bulk institutional orders with advance written notification

Strict Production & Batch Inspection Quality Control Standards All HCG glycoprotein cell signal solid reagents are produced and inspected in accordance with unified international biological laboratory protein reagent testing specifications. Each separate production batch completes comprehensive multi-index full inspection, with signed official exclusive batch inspection reports delivered alongside every customer order. All inspection operation standards comply with USP <1231> biological reagent testing guidelines, ICH Q3A impurity control rules and ISO 17034 analytical laboratory quality specifications. Core High Purity Benchmark Finished heterodimer glycoprotein solid chromatographic purity ≥98.0% verified by single-method reversed-phase HPLC testing; complete chromatogram image records are attached to each batch inspection report. Single unknown individual impurity ≤0.3%, total sum of all unknown impurities ≤1.0%. C18 reversed-phase chromatographic column with 214nm UV wavelength detection is adopted for accurate purity measurement, simultaneously verifying uniformity of glycosylation structure and residual volume of split free subunit fragments. Mandatory Full Batch Inspection Items Mass spectrometry molecular weight matching testing, deviation between measured molecular weight of complete heterodimer glycoprotein and theoretical standard value controlled within ±150 Da Full peptide mapping structural conformation verification to confirm complete matching with standard native HCG heterodimer glycosylation molecular configuration Trace residual eukaryotic host protein and purification auxiliary reagent impurity testing complying with ICH Q3C standardized residual limit requirements Constant temperature & humidity accelerated stability testing for shelf-life validity confirmation Quantitative consistency testing of LHCGR receptor binding efficiency and gonadal cell steroid metabolism regulatory biological activity 100% manual visual full inspection of all sealed finished vials before shipment to screen defective products with abnormal appearance or incomplete vacuum sealing Complete batch full traceability management system covering eukaryotic cell expression raw material logs, purification production operation records, full-item inspection data and outgoing delivery archive files

Standard Step-by-Step Dissolving Operation Guidance Recommended Matching Solvent Medium: Laboratory dedicated bacteriostatic purified water / pH 7.0–7.6 neutral PBS buffer

Storage Environment & Valid Usage Cycle Regulatory Specifications Unopened Original Vacuum-Sealed Vials Long-term sealed storage condition: Store fully sealed vials at -20℃ ~ -80℃, completely shielded from direct sunlight and high ambient humidity (photosensitive and hygroscopic glycosylated glycoprotein must avoid repeated exposure to humid air and strong light irradiation) Valid stable storage period under constant -20℃ freezing environment: 12 months Valid stable storage period under constant -80℃ ultra-low temperature environment: 36 months Short-distance transportation stability tolerance: Retain complete receptor binding signal activity for up to 7 days under ambient temperature ≤25℃, avoid continuous strong light exposure during transit Forbidden storage operations: Frequent repeated temperature cycle fluctuation, long-time continuous direct sunlight exposure, storage adjacent to volatile oxidizing organic chemical reagents, repeated opening of sealed vial under humid atmospheric environment Liquid Mixed With Bacteriostatic Purified Water / PBS Buffer After Full Dissolution Storage condition after complete dissolution: Place inside refrigerator under 2℃ ~ 8℃ refrigeration environment, fully shaded from light and tightly sealed Suggested full consumption time limit: Use up all mixed liquid within 14 days after preparation completion Strict prohibition rule: Do not freeze diluted mixed liquid; repeated freeze-thaw cycles will permanently damage glycoprotein heterodimer skeleton and glycosylation coordination stability, completely losing receptor signal regulatory activity for reproductive endocrine cell culture research

Shipping & Receiving Handling Reminders Small trial vial orders can be delivered via standard international express for short-distance cross-border transportation routes, equipped with light-proof outer packaging Large multi-vial batch orders are highly recommended to adopt temperature-insulated foam protective packaging fitted with ice packs and full light-proof outer bags to maintain stable molecular structure of heterodimer glycoprotein during long-distance overseas transportation Complete supporting shipping documents will be delivered together with all parcels, including unified technical datasheets, batch exclusive COA test records and molecular glycosylation structural verification files, for customs inspection and internal laboratory filing use

Laboratory Operation Safety Precautions Wear disposable nitrile protective gloves, transparent impact-resistant safety goggles and dust-proof laboratory masks during solid powder weighing and liquid dissolving operation processes Avoid direct skin contact, eye mucous membrane contact and inhalation of dry freeze-dried glycoprotein cell signal solid powder In case of accidental contact with skin or eye tissue, immediately flush the affected area thoroughly with abundant clean running water continuously for no less than 5 minutes Dispose residual solid powder and waste mixed liquid strictly following internal biosafety waste management rules of your laboratory; never pour waste liquid directly into domestic drainage pipelines or ordinary household garbage bins

Important Usage Restriction & Disclaimer Statement This HCG heterodimer glycosylated glycoprotein receptor signal solid reagent is exclusively developed for fundamental scientific research projects of universities, biomedical research institutes and biotechnology R&D teams. This product is not medical supplies, therapeutic raw materials or cosmetic ingredient raw materials, and has not obtained official regulatory authorization from global supervision authorities for any human body intervention use, oral intake or animal disease diagnosis and treatment. Resale to individual private users for personal physical conditioning or unregulated self-use is strictly prohibited. Any human-related experimental research can only be carried out after obtaining formal written regulatory approval documents.

Free Supporting Technical Documents Provided Upon All Shipment Order Completion After order shipment completion, all customers can receive a full set of free complete technical archive files: